| Preparation and characteristics of antibodies to a sulfur-containing hapten, mimicking the transition state in lactamization reactions | |
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MedLine Citation:
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PMID: 9493252 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sulfur-containing hapten was proposed to be an analogue of the tetrahedral transition state of lactamization reactions. The dynamics of the immune response to this hapten upon using different immunization schemes was studied. Sixty-one clones of mouse anti-hapten antibodies were obtained. Three clones efficiently bound not only the transition state analogue (the hapten) but also potential substrates of lactamization reactions. Antibody-induced changes in the optical absorption spectra of substrate solutions were studied for a substrate with stabilized active conformation (2-aminomethylbenzoate) and an unmodified substrate (gamma-aminobutyric acid), which demonstrated that the catalytic antibodies obtained significantly accelerated the lactam ring formation. |
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Authors:
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A V Zherdev; B B Dzantiev; V O Popov; L B Dzantiev; A J Kirby; V V Sviridov; I V Iakovleva |
Publication Detail:
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Type: English Abstract; Journal Article |
Journal Detail:
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Title: Prikladnaia biokhimiia i mikrobiologiia Volume: 33 ISSN: 0555-1099 ISO Abbreviation: Prikl. Biokhim. Mikrobiol. Publication Date: 1997 Nov-Dec |
Date Detail:
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Created Date: 1998-04-03 Completed Date: 1998-04-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0023416 Medline TA: Prikl Biokhim Mikrobiol Country: RUSSIA |
Other Details:
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Languages: rus Pagination: 624-31 Citation Subset: IM |
Affiliation:
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Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia. |
Vernacular Title:
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Poluchenie i kharakteristika antitel k serosoderzhashchemu gaptenu, imitiruiushchemu perekhodnoe sostoianie v reaktsiiakh laktamizatsii. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology* Benzoates / chemistry, immunology Binding Sites, Antibody Fluorescent Antibody Technique Haptens / chemistry, immunology* Lactams / chemistry* Mice Molecular Conformation Molecular Mimicry Spectrum Analysis Sulfur / analysis* gamma-Aminobutyric Acid / chemistry, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Benzoates; 0/Binding Sites, Antibody; 0/Haptens; 0/Lactams; 56-12-2/gamma-Aminobutyric Acid; 7704-34-9/Sulfur |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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