Document Detail


Preparation and characteristics of antibodies to a sulfur-containing hapten, mimicking the transition state in lactamization reactions
MedLine Citation:
PMID:  9493252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A sulfur-containing hapten was proposed to be an analogue of the tetrahedral transition state of lactamization reactions. The dynamics of the immune response to this hapten upon using different immunization schemes was studied. Sixty-one clones of mouse anti-hapten antibodies were obtained. Three clones efficiently bound not only the transition state analogue (the hapten) but also potential substrates of lactamization reactions. Antibody-induced changes in the optical absorption spectra of substrate solutions were studied for a substrate with stabilized active conformation (2-aminomethylbenzoate) and an unmodified substrate (gamma-aminobutyric acid), which demonstrated that the catalytic antibodies obtained significantly accelerated the lactam ring formation.
Authors:
A V Zherdev; B B Dzantiev; V O Popov; L B Dzantiev; A J Kirby; V V Sviridov; I V Iakovleva
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Prikladnaia biokhimiia i mikrobiologiia     Volume:  33     ISSN:  0555-1099     ISO Abbreviation:  Prikl. Biokhim. Mikrobiol.     Publication Date:    1997 Nov-Dec
Date Detail:
Created Date:  1998-04-03     Completed Date:  1998-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0023416     Medline TA:  Prikl Biokhim Mikrobiol     Country:  RUSSIA    
Other Details:
Languages:  rus     Pagination:  624-31     Citation Subset:  IM    
Affiliation:
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Vernacular Title:
Poluchenie i kharakteristika antitel k serosoderzhashchemu gaptenu, imitiruiushchemu perekhodnoe sostoianie v reaktsiiakh laktamizatsii.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology*
Benzoates / chemistry,  immunology
Binding Sites, Antibody
Fluorescent Antibody Technique
Haptens / chemistry,  immunology*
Lactams / chemistry*
Mice
Molecular Conformation
Molecular Mimicry
Spectrum Analysis
Sulfur / analysis*
gamma-Aminobutyric Acid / chemistry,  immunology
Chemical
Reg. No./Substance:
0/Antibodies; 0/Benzoates; 0/Binding Sites, Antibody; 0/Haptens; 0/Lactams; 56-12-2/gamma-Aminobutyric Acid; 7704-34-9/Sulfur

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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