| Preparation and characterisation of oligosaccharides produced by nitrous acid depolymerisation of chitosans. | |
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MedLine Citation:
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PMID: 11448674 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two chitosans with widely different chemical composition (fraction of N-acetylated units (F(A))<0.001 and F(A)=0.59), were degraded by nitrous acid, to obtain the reactive 2,5-anhydro-D-mannose- (M-) unit at the new reducing end. The fully N-acetylated and fully N-deacetylated oligomers were separated by size-exclusion chromatography. Both the chemical structure and purity were studied by one- and two-dimensional 1H and 13C NMR methods. The fully N-acetylated oligomers were found to be stable, whereas the N-deacetylated oligomers reacted intermolecularly by a Schiff base reaction between the 2-amino group on the N-deacetylated units and the M-units, facilitating the cleavage of the glycosidic bond next to the M-unit and the formation of 5-hydroxymethylfurfural (HMF). |
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Authors:
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K Tømmeraas; K M Vårum; B E Christensen; O Smidsrød |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carbohydrate research Volume: 333 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-07-12 Completed Date: 2001-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 137-44 Citation Subset: IM |
Affiliation:
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Norwegian Biopolymer Laboratory (NOBIPOL), Department of Biotechnology, Norwegian University of Science and Technology (NTNU), N-7491, Trondheim, Norway. kt@chembio.ntnu.no |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Carbohydrate Sequence Chitin / analogs & derivatives, chemistry*, metabolism Chitosan Chromatography / methods Magnetic Resonance Spectroscopy Molecular Sequence Data Molecular Structure Nitrous Acid / chemistry* Oligosaccharides / chemistry*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides; 1398-61-4/Chitin; 7782-77-6/Nitrous Acid; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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