Document Detail


Preparation of cell populations with stabilized erythropoietic potential from the primitive streak chick blastodisc: some implications for control of gastrulation.
MedLine Citation:
PMID:  208727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Improved methods for preparation from primitive streak chick blastodiscs of cell suspensions capable of forming erythroid cells in culture have been developed. When blastodiscs were preincubayed with hyaluronidase in the absence of collagenase before cell dispersion and a high concentration of methyl-alpha-mannoside was present in all media, the yields of cells were some 10-fold higher than those obtained by former procedures. Cell suspensions obtained consisted almost entirely of viable cells, yielded large numbers of free mature erythrocytes in liquid culture, and formed erythroid colonies and bursts in solidified medium. The capacity to form differentiated cells after resedimenrtation through Ficoll density gradients was partly stabilized. Addition of gee yolk homogenate to the blastodiscs immediately following treatment with hyaluronidase and to all media used thereafter largely stabilized the capacity to form erythroid cells during resedimentation through Ficoll density gradients. Possible relevance of observations made during development of the procedures to the control of onset of cell migration in the process of gastrulation is indicated.
Authors:
S D Wainwright; L K Wainwright
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of biochemistry     Volume:  56     ISSN:  0008-4018     ISO Abbreviation:  Can. J. Biochem.     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-09-25     Completed Date:  1978-09-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0421034     Medline TA:  Can J Biochem     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  430-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Differentiation / drug effects
Cell Movement
Cell Separation / methods
Cells, Cultured
Chick Embryo / cytology*
Egg Yolk / physiology
Erythropoiesis / drug effects
Female
Hematopoietic Stem Cells / cytology*
Hyaluronoglucosaminidase / metabolism
Methylmannosides / pharmacology
Microbial Collagenase / metabolism
Chemical
Reg. No./Substance:
0/Methylmannosides; EC 3.2.1.35/Hyaluronoglucosaminidase; EC 3.4.24.3/Microbial Collagenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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