| Preparation and biological activity of molecular probes to identify and analyze jasmonic acid-binding proteins. | |
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MedLine Citation:
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PMID: 15277750 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several types of jasomonic acid (JA) derivatives, including JA--amino acid conjugates, a JA--biotin conjugate, a JA--dexamethasone heterodimer, and a JA-fluoresceine conjugate, were prepared as candidates for molecular probes to identify JA--binding proteins. These JA derivatives, excepting the JA--fluoresceine conjugate, exhibited significant biological activities in a rice seedling assay, a rice phytoalexin-inducing assay, and/or a soybean phenylalanine ammonia-lyase-inducing assay. These JA derivatives could therefore be useful probes for identifying JA--binding proteins. The activity spectra of the prepared compounds were different from each other, suggesting that different types of JA receptors were involved in the perception of JA derivatives in the respective bioassays. |
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Authors:
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Yusuke Jikumaru; Tadao Asami; Hideharu Seto; Shigeo Yoshida; Tadashi Yokoyama; Naomi Obara; Morifumi Hasegawa; Osamu Kodama; Makoto Nishiyama; Kazunori Okada; Hideaki Nojiri; Hisakazu Yamane |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 68 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-27 Completed Date: 2005-03-29 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 1461-6 Citation Subset: IM |
Affiliation:
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Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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chemistry* Biotin / analogs & derivatives*, chemistry Cyclopentanes / chemical synthesis, chemistry, metabolism* Dexamethasone / analogs & derivatives*, chemistry Fluoresceins / chemistry* Molecular Probes / chemical synthesis*, metabolism Oryza sativa / metabolism Oxylipins Phenylalanine Ammonia-Lyase / metabolism Plant Extracts / metabolism Protein Binding Soybeans / metabolism Terpenes |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Cyclopentanes; 0/Fluoresceins; 0/Molecular Probes; 0/Oxylipins; 0/Plant Extracts; 0/Terpenes; 37297-20-4/phytoalexins; 50-02-2/Dexamethasone; 58-85-5/Biotin; 6894-38-8/jasmonic acid; EC 4.3.1.24/Phenylalanine Ammonia-Lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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