Document Detail


Preparation and biological activity of molecular probes to identify and analyze jasmonic acid-binding proteins.
MedLine Citation:
PMID:  15277750     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several types of jasomonic acid (JA) derivatives, including JA--amino acid conjugates, a JA--biotin conjugate, a JA--dexamethasone heterodimer, and a JA-fluoresceine conjugate, were prepared as candidates for molecular probes to identify JA--binding proteins. These JA derivatives, excepting the JA--fluoresceine conjugate, exhibited significant biological activities in a rice seedling assay, a rice phytoalexin-inducing assay, and/or a soybean phenylalanine ammonia-lyase-inducing assay. These JA derivatives could therefore be useful probes for identifying JA--binding proteins. The activity spectra of the prepared compounds were different from each other, suggesting that different types of JA receptors were involved in the perception of JA derivatives in the respective bioassays.
Authors:
Yusuke Jikumaru; Tadao Asami; Hideharu Seto; Shigeo Yoshida; Tadashi Yokoyama; Naomi Obara; Morifumi Hasegawa; Osamu Kodama; Makoto Nishiyama; Kazunori Okada; Hideaki Nojiri; Hisakazu Yamane
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  68     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-27     Completed Date:  2005-03-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1461-6     Citation Subset:  IM    
Affiliation:
Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / chemistry*
Biotin / analogs & derivatives*,  chemistry
Cyclopentanes / chemical synthesis,  chemistry,  metabolism*
Dexamethasone / analogs & derivatives*,  chemistry
Fluoresceins / chemistry*
Molecular Probes / chemical synthesis*,  metabolism
Oryza sativa / metabolism
Oxylipins
Phenylalanine Ammonia-Lyase / metabolism
Plant Extracts / metabolism
Protein Binding
Soybeans / metabolism
Terpenes
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Cyclopentanes; 0/Fluoresceins; 0/Molecular Probes; 0/Oxylipins; 0/Plant Extracts; 0/Terpenes; 37297-20-4/phytoalexins; 50-02-2/Dexamethasone; 58-85-5/Biotin; 6894-38-8/jasmonic acid; EC 4.3.1.24/Phenylalanine Ammonia-Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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