Document Detail


Preparation and biodisposition of methoxypolyethylene glycol amine-poly(DL-lactic acid) copolymer nanoparticles loaded with pyrene-ended poly(DL-lactic acid).
MedLine Citation:
PMID:  18448290     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A formyl group-ended poly(DL-lactic acid) (PLA-aldehyde), synthesized in the same manner as reported previously, was utilized to produce the polymeric marker for PLA-related nanoparticles. Namely, pyrene-ended poly(DL-lactic acid) (PLA-pyrene) was prepared as a polymeric marker by the reductive amination of PLA-aldehyde and aminopyrene. Methoxypolyethylene glycol amine-poly(DL-lactic acid) block copolymer (PLA-(MeO-PEG) nanoparticles loaded with PLA-pyrene were prepared, and examined on retention of PLA-pyrene in the nanoparticles, and biodisposition in normal and sarcoma-180 solid tumor-bearing mice. PLA-pyrene was retained stably in PLA-(MeO-PEG) nanoparticles in a PBS-ethanol (7:3, v/v) mixture and a plasma-PBS (1:1, v/v) mixture, indicating that PLA-pyrene might be a useful marker of PLA-(MeO-PEG) nanoparticles themselves. After i.v. injection in normal rats, the plasma level of PLA-pyrene was very high for initial 8h, and accumulated gradually into organs, especially spleen and liver. After i.v. injection in tumor-bearing mice, similar biodistribution profiles of PLA-pyrene were observed, and PLA-pyrene was accumulated well in tumor, suggesting that PLA-(MeO-PEG) nanoparticles should be delivered efficiently to solid tumors. It is suggested that PLA-pyrene might be a useful probe of the nanoparticles themselves. In addition, it was demonstrated that PLA-(MeO-PEG) nanoparticles should be a useful drug carrier for passive tumor targeting.
Authors:
Masanaho Sasatsu; Hiraku Onishi; Yoshiharu Machida
Publication Detail:
Type:  Journal Article     Date:  2008-03-18
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  358     ISSN:  0378-5173     ISO Abbreviation:  Int J Pharm     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-03     Completed Date:  2008-09-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  271-7     Citation Subset:  IM    
Affiliation:
Department of Drug Delivery Research, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Gel
Fluorescent Dyes
Indicators and Reagents
Injections, Intravenous
Lactic Acid / chemical synthesis,  chemistry*,  pharmacokinetics
Magnetic Resonance Spectroscopy
Male
Mice
Microscopy, Electron, Transmission
Nanoparticles / chemistry*
Particle Size
Polyethylene Glycols / chemical synthesis,  chemistry*,  pharmacokinetics
Polymers / chemical synthesis,  chemistry*,  pharmacokinetics
Pyrenes / chemical synthesis,  chemistry*,  pharmacokinetics
Sarcoma 180 / metabolism
Tissue Distribution
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Indicators and Reagents; 0/Polyethylene Glycols; 0/Polymers; 0/Pyrenes; 26100-51-6/poly(lactic acid); 50-21-5/Lactic Acid; 9004-74-4/monomethoxypolyethylene glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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