Document Detail


Preparation and application of a fluorogenic substrate for endo-beta-xylosidase.
MedLine Citation:
PMID:  2511235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present paper describes a fluorometric assay for galactosaminoglycan-degrading endo-beta-xylosidase, utilizing glycosaminoglycan chains bearing a 4-methylumbelliferyl group at the reducing terminus as a substrate. This fluorogenic substrate is synthesized by human skin fibroblasts cultured in the presence of a fluorogenic xyloside, 4-methylumbelliferyl-beta-D-xyloside. The assay is based on measurement of the fluorescence of 4-methylumbelliferone, enzymatically liberated from the synthetic substrate by endo-beta-xylosidase. We examined the applicability of the assay for analysis of endo-beta-xylosidase activity.
Authors:
K Takagaki; A Kon; H Kawasaki; T Nakamura; M Endo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  19     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:    1989 Aug-Sep
Date Detail:
Created Date:  1990-01-11     Completed Date:  1990-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  207-14     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Hirosaki University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Electrophoresis, Cellulose Acetate / methods
Fibroblasts / enzymology
Glycoside Hydrolases / analysis*
Humans
Hymecromone / analogs & derivatives,  analysis
Indicators and Reagents
Kinetics
Liver / enzymology
Rabbits
Skin / enzymology
Xylosidases / analysis*,  metabolism
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 6734-33-4/methylumbelliferyl-beta-D-xyloside; 90-33-5/Hymecromone; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.-/Xylosidases; EC 3.2.1.-/endo-beta-xylosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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