Document Detail

Preparation of anti-pefloxacin antibody and development of an indirect competitive enzyme-linked immunosorbent assay for detection of pefloxacin residue in chicken liver.
MedLine Citation:
PMID:  16968054     Owner:  NLM     Status:  MEDLINE    
Pefloxacin has been increasingly used in veterinary medicine to treat microbial infections. To avoid using a labor-intensive instrumental method to detect the residue of pefloxacin in food, a simple and convenient indirect competitive enzyme-linked immunosorbent assay method has been developed in this study. The antibody generated from immunogen cationized bovine serum albumin-pefloxacin showed high sensitivity toward pefloxacin with an IC50 value of 6.7 ppb in buffer and was suitable for a screening assay to detect the residue of pefloxacin in food products. The antibody has been assessed using rapid enzyme immunoassays to exploit its specificity. The antibody prepared shows cross-reactivity with a few other (fluoro)quinolones including fleroxacin (116%), enrofloxacin (88%), and ofloxacin (10%). The assay measured drug residue in chicken liver spiked with pefloxacin with an interassay coefficient of variation of 13.6% or less and an intra-assay coefficient of variation of 10.9% or less. The average recovery rates at 0.5, 5, 10, 50, and 100 ppb were in the range of 86-106% for interassay and in the range of 87-103% for intra-assay, respectively.
Shengxin Lu; Yulan Zhang; Jingting Liu; Chengbiao Zhao; Wei Liu; Rimo Xi
Related Documents :
16927394 - On-farm management practices to minimise off-site movement of pesticides from furrow ir...
19533104 - Analysis of antibiotics in fish samples.
23865184 - Combination of plant and insect eggs as food sources facilitates ovarian development in...
8920144 - Organohalogen and organophosphorus pesticides in mixed feed rations: findings from fda'...
23588464 - Nootropic and hypophagic effects following long term intake of almonds (prunus amygdalu...
15221354 - Passclaim--diet-related cancer.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  54     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-13     Completed Date:  2006-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6995-7000     Citation Subset:  IM    
The School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibodies / immunology*
Antibody Specificity
Binding, Competitive
Drug Residues / analysis
Enzyme-Linked Immunosorbent Assay / methods*
Liver / chemistry*
Pefloxacin / analysis*,  immunology*
Sensitivity and Specificity
Reg. No./Substance:
0/Antibodies; 70458-92-3/Pefloxacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Champagne experiences various rhythmical bubbling regimes in a flute.
Next Document:  Rapid sample preparation method for LC-MS/MS or GC-MS analysis of acrylamide in various food matrice...