Document Detail


Preparation of anti-pefloxacin antibody and development of an indirect competitive enzyme-linked immunosorbent assay for detection of pefloxacin residue in chicken liver.
MedLine Citation:
PMID:  16968054     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pefloxacin has been increasingly used in veterinary medicine to treat microbial infections. To avoid using a labor-intensive instrumental method to detect the residue of pefloxacin in food, a simple and convenient indirect competitive enzyme-linked immunosorbent assay method has been developed in this study. The antibody generated from immunogen cationized bovine serum albumin-pefloxacin showed high sensitivity toward pefloxacin with an IC50 value of 6.7 ppb in buffer and was suitable for a screening assay to detect the residue of pefloxacin in food products. The antibody has been assessed using rapid enzyme immunoassays to exploit its specificity. The antibody prepared shows cross-reactivity with a few other (fluoro)quinolones including fleroxacin (116%), enrofloxacin (88%), and ofloxacin (10%). The assay measured drug residue in chicken liver spiked with pefloxacin with an interassay coefficient of variation of 13.6% or less and an intra-assay coefficient of variation of 10.9% or less. The average recovery rates at 0.5, 5, 10, 50, and 100 ppb were in the range of 86-106% for interassay and in the range of 87-103% for intra-assay, respectively.
Authors:
Shengxin Lu; Yulan Zhang; Jingting Liu; Chengbiao Zhao; Wei Liu; Rimo Xi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  54     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-13     Completed Date:  2006-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6995-7000     Citation Subset:  IM    
Affiliation:
The School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology*
Antibody Specificity
Binding, Competitive
Chickens*
Drug Residues / analysis
Enzyme-Linked Immunosorbent Assay / methods*
Liver / chemistry*
Male
Pefloxacin / analysis*,  immunology*
Rabbits
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antibodies; 70458-92-3/Pefloxacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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