Document Detail


THE PREPARATION OF ADENOSINE 5'-PYROPHOSPHATE BY A NON-ENZYMIC METHOD.
MedLine Citation:
PMID:  14333545     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. A non-enzymic method for the preparation of adenosine 5'-diphosphate is described, in which the terminal phosphate of adenosine 5'-triphosphate is transferred to methanol in the presence of hydrochloric acid. The final purified product can be obtained in 60% yield. 2. Experiments with [(14)C]methanol showed that no methylation of the adenosine diphosphate occurs during the reaction. 3. Confirmation that the pyrophosphate moiety of the adenosine diphosphate produced was in the 5'-position was obtained by: (a) periodate oxidation; (b) treatment with apyrase and examination of the resulting adenylic acid isomer by paper chromatography. 4. The method appears to be generally applicable to the preparation of nucleoside 5'-diphosphates from the corresponding nucleoside 5'-triphosphates.
Authors:
R M DAWSON; M FORD; J EICHBERG
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  95     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1965 Apr 
Date Detail:
Created Date:  1965-11-01     Completed Date:  1996-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  104-6     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Adenine Nucleotides*
Adenosine Triphosphate*
Chemical Phenomena
Chemistry*
Chromatography*
Methanol*
Periodic Acid*
Research*
Chemical
Reg. No./Substance:
0/Adenine Nucleotides; 10450-60-9/Periodic Acid; 56-65-5/Adenosine Triphosphate; 67-56-1/Methanol
Comments/Corrections

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