Document Detail


Preparation and properties of chloroplasts depleted of chloroplast coupling factor 1 by sodium bromide treatment.
MedLine Citation:
PMID:  16659067     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Chloroplasts were treated with 2 m sodium bromide. The resulting particles lost their ATPase activity and chloroplast coupling factor 1 subunits were detected in the supernatant by means of gel electrophoresis and specific antibodies. The chloroplast coupling factor 1 depleted particles show high rates of Hill reaction with pH optimum shifted toward lower pH. The sodium bromide treatment also abolished the light-induced proton uptake. In the presence of N-methylphenazonium methosulfate light-induced proton release, insensitive to uncouplers, was observed. Addition of dicyclohexylcarbodiimide reversed the light-induced pH changes to the normal proton uptake and increased the pH optimum of the Hill reaction.
Authors:
A Kamienietzky; N Nelson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  55     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1975 Feb 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  282-7     Citation Subset:  -    
Affiliation:
Department of Biology, Technicon-Israel Institute of Technology, Haifa, Israel.
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