Document Detail


Preparation and orcein staining of mitotic chromosomes from Drosophila larval brain.
MedLine Citation:
PMID:  20194458     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this protocol, larval brains from Drosophila are incubated in vitro with colchicine, treated with hypotonic solution, fixed, and squashed in aceto-orcein. This procedure provides a large number of well-spread metaphase figures (200-400 per brain) that can be analyzed for chromosome morphology, the presence of chromosome aberrations, and the degree of ploidy.
Authors:
Sergio Pimpinelli; Silvia Bonaccorsi; Laura Fanti; Maurizio Gatti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cold Spring Harbor protocols     Volume:  2010     ISSN:  1559-6095     ISO Abbreviation:  Cold Spring Harb Protoc     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-23     Completed Date:  2010-07-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101524530     Medline TA:  Cold Spring Harb Protoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  pdb.prot5389     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / cytology
Chromosomes*
Drosophila / cytology*
Fluorescent Dyes*
Larva / cytology
Mitosis*
Oxazines*
Staining and Labeling / methods*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Oxazines; 1400-62-0/PAcein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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