Document Detail

Preparation and orcein staining of mitotic chromosomes from Drosophila larval brain.
MedLine Citation:
PMID:  20194458     Owner:  NLM     Status:  MEDLINE    
In this protocol, larval brains from Drosophila are incubated in vitro with colchicine, treated with hypotonic solution, fixed, and squashed in aceto-orcein. This procedure provides a large number of well-spread metaphase figures (200-400 per brain) that can be analyzed for chromosome morphology, the presence of chromosome aberrations, and the degree of ploidy.
Sergio Pimpinelli; Silvia Bonaccorsi; Laura Fanti; Maurizio Gatti
Related Documents :
12113628 - Simultaneous collection of topographic and fluorescent images of barley chromosomes by ...
2050548 - X-ray microanalysis of toluidine blue stained chromosomes: a quantitative study of the ...
378618 - A study of heterochromatin in drosophila nasuta by the 5-bromodeoxyuridine-giemsa stain...
25091548 - Paternal inheritance in mealybugs (hemiptera: coccoidea: pseudococcidae).
7513888 - Co-amplification of the cystic fibrosis delta f508 mutation with the hla dqa1 sequence ...
1330828 - The distribution of genes on human chromosomes as studied by in situ nick translation.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cold Spring Harbor protocols     Volume:  2010     ISSN:  1559-6095     ISO Abbreviation:  Cold Spring Harb Protoc     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-23     Completed Date:  2010-07-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101524530     Medline TA:  Cold Spring Harb Protoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  pdb.prot5389     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Brain / cytology
Drosophila / cytology*
Fluorescent Dyes*
Larva / cytology
Staining and Labeling / methods*
Reg. No./Substance:
0/Fluorescent Dyes; 0/Oxazines; 1400-62-0/PAcein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Suspended Embryo Mount for Imaging Caenorhabditis elegans.
Next Document:  Chromosome Banding of Mitotic Chromosomes from Drosophila Larval Brain.