Document Detail

Preparation of monoclonal antibody for melamine and development of an indirect competitive ELISA for melamine detection in raw milk, milk powder, and animal feeds.
MedLine Citation:
PMID:  20593820     Owner:  NLM     Status:  MEDLINE    
Melamine (MEL) has been involved in several food recalls after the discovery of severe kidney damages in children and pets poisoned by melamine-adulterated food. To detect MEL residue in foods and animal feeds, an indirect competitive ELISA (cELISA) method was developed in this study based on preparation of monoclonal antibodies (MAbs) to MEL. The immunogen was prepared by linking MEL hapten with carrier protein via carbodiimide method. The method is applicable in the range of 5.0-135.0 microg L(-1) MEL in buffer solution, with an IC(50) value of 22.6 +/- 1.9 microg L(-1). The MAbs showed high specificity with low cross-reactivity (< or =1%) toward cyanurate, ammelide, and ammeline. The method was utilized in the detection of MEL in raw milk, milk powder, and animal feeds, with detection limits of 0.1 mg L(-1) for milk, 0.2 mg kg(-1) for milk powder, and 0.5 mg L(-1) for feeds. The recovery ratio was 79-110% for all matrices. The intra-assay and interassay coefficients of variation were <12.0 and <13.0%, respectively. Finally, the application of the cELISA in quantity evaluation of MEL in various feeds from local markets was evaluated and discussed.
Weiwei Yin; Jinting Liu; Taichang Zhang; Weihua Li; Wei Liu; Meng Meng; Fangyang He; Yuping Wan; Caiwei Feng; Shanliang Wang; Xiao Lu; Rimo Xi
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-29     Completed Date:  2010-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8152-7     Citation Subset:  IM    
The School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong, People's Republic of China.
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MeSH Terms
Animal Feed / analysis*
Antibodies, Monoclonal / analysis*
Enzyme-Linked Immunosorbent Assay / methods*
Food Contamination / analysis*
Mice, Inbred BALB C
Milk / chemistry*
Triazines / analysis*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Triazines; 108-78-1/melamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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