| Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis. | |
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MedLine Citation:
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PMID: 18370016 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chromosomes are supermolecules that contain most of the DNA within a cell and are visible under optical and electron microscopes. Although they were observed at the earliest stage of genetics, their fundamental structure is not yet understood. The reasons for this are debated among researchers; however, it is clear that the accumulation of metaphase chromosomes for their biochemical analysis has been a significant challenge. In this chapter, a method is described for accumulating and preparing human metaphase chromosomes in sufficient amount to perform their proteome analysis. Preparation and separation methods of chromosome proteins are described, followed by a protocol for their identification by mass spectrometry. |
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Authors:
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Kiichi Fukui; Hideaki Takata; Susumu Uchiyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 432 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-03-28 Completed Date: 2008-08-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 149-60 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cell Membrane / ultrastructure Chromosomal Proteins, Non-Histone / chemistry, isolation & purification Chromosomes, Human / chemistry*, ultrastructure* Demecolcine Electrophoresis, Gel, Two-Dimensional / methods Electrophoresis, Polyacrylamide Gel / methods Hela Cells Humans Indicators and Reagents Mass Spectrometry / methods Nuclear Proteins / chemistry*, isolation & purification* Proteome* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods |
| Chemical | |
Reg. No./Substance:
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0/Chromosomal Proteins, Non-Histone; 0/Indicators and Reagents; 0/Nuclear Proteins; 0/Proteome; 477-30-5/Demecolcine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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