Document Detail

Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis.
MedLine Citation:
PMID:  18370016     Owner:  NLM     Status:  MEDLINE    
Chromosomes are supermolecules that contain most of the DNA within a cell and are visible under optical and electron microscopes. Although they were observed at the earliest stage of genetics, their fundamental structure is not yet understood. The reasons for this are debated among researchers; however, it is clear that the accumulation of metaphase chromosomes for their biochemical analysis has been a significant challenge. In this chapter, a method is described for accumulating and preparing human metaphase chromosomes in sufficient amount to perform their proteome analysis. Preparation and separation methods of chromosome proteins are described, followed by a protocol for their identification by mass spectrometry.
Kiichi Fukui; Hideaki Takata; Susumu Uchiyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  432     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-03-28     Completed Date:  2008-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  149-60     Citation Subset:  IM    
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan.
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MeSH Terms
Cell Line
Cell Membrane / ultrastructure
Chromosomal Proteins, Non-Histone / chemistry,  isolation & purification
Chromosomes, Human / chemistry*,  ultrastructure*
Electrophoresis, Gel, Two-Dimensional / methods
Electrophoresis, Polyacrylamide Gel / methods
Hela Cells
Indicators and Reagents
Mass Spectrometry / methods
Nuclear Proteins / chemistry*,  isolation & purification*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Reg. No./Substance:
0/Chromosomal Proteins, Non-Histone; 0/Indicators and Reagents; 0/Nuclear Proteins; 0/Proteome; 477-30-5/Demecolcine

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