Document Detail

Preparation of Mammalian oocytes for transmission electron microscopy.
MedLine Citation:
PMID:  23138955     Owner:  NLM     Status:  In-Data-Review    
The visualization of subcellular organelles and structures is a valuable tool for understanding cellular changes that occur in oocytes and early embryos as a result of genetic alterations, incubation conditions, drug treatments, and many other manipulations. Preparing oocytes for transmission electron microscopic analysis can be challenging as these cells cannot be visualized without a microscope and they are more susceptible to mechanical disruption during manipulation. Here we describe methods for immobilizing oocytes on either a solid surface or within a matrix and then document our embedding techniques which work well for preserving the ultrastructure of the mouse oocyte.
Lynne Anguish; Scott Coonrod
Related Documents :
16851975 - Intermolecular potential to represent collisions of protonated peptide ions with fluori...
22039995 - Charge transfer events in semiconducting single-wall carbon nanotubes.
19096885 - Conformational and electronic (aim/nbo) study of unsubstituted a-type dimeric proanthoc...
16861515 - A solution for using dynamic data sets in electronic presentations.
23838525 - Paramagnetic electrodes and bulk magnetic susceptibility effects in the in situ nmr stu...
17388265 - Thiamin deprotonation mechanism. carbanion development stabilized by the lumos of thiaz...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  213-30     Citation Subset:  IM    
Baker Institute for Animal Health, Cornell University, Ithaca, NY, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Chromosome spreads with centromere staining in mouse oocytes.
Next Document:  Measuring ca(2+) oscillations in Mammalian eggs.