Document Detail


Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry.
MedLine Citation:
PMID:  24182927     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The purpose of this protocol is to take any biopsy or whole organ tissue from animals or human, formalin-fix the specimen to preserve the current state of the tissue, and embed it into a paraffin block and for future immunohistochemistry experiments (If you intend to fix cells, check the alternative protocols: Preparation of Cells for Microscopy using Cytospin, Preparation of Cells for Microscopy using Chamber Slides and Coverslips, or Preparation of Cells for Microscopy using 'Cell Blocks').
Authors:
Kirstie Canene-Adams
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  533     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-11-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  225-33     Citation Subset:  IM    
Copyright Information:
© 2013 Elsevier Inc. All rights reserved.
Affiliation:
Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA. Electronic address: kirstieadams26@yahoo.com.
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