Document Detail


Preparation and epitope mapping of a monoclonal antibody against Tri a Bd 27K, a major wheat allergen.
MedLine Citation:
PMID:  19734669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We produced a monoclonal antibody (mAb) as a probe for detection of Tri a Bd 27K, a major wheat allergen. The mAb recognized the allergen purified from wheat flour, and the epitope on the allergen to the mAb was determined to be amino acid sequence (154)VPWVVVDGKPL(164) of Tri a Bd 27K. Of the amino acid residues on the epitope, the amino acid residues responsible for the binding to the mAb were found to be W156, D160, G161, and P163.
Authors:
Nobuko Komiyama; Miki Hiemori; Masumi Kimoto; Makiko Suzuki; Hiromi Yamashita; Yoshitaka Takahashi; Hideaki Tsuji
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Publication Detail:
Type:  Journal Article     Date:  2009-09-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  73     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-24     Completed Date:  2009-12-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2113-6     Citation Subset:  IM    
Affiliation:
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Antibodies, Monoclonal / chemistry,  immunology*
Antigens, Plant / immunology*
Base Sequence
Blotting, Western
DNA Primers
Electrophoresis, Polyacrylamide Gel
Epitope Mapping*
Polymerase Chain Reaction
Triticum / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Plant; 0/DNA Primers; 0/Tri a Bd 27K allergen, Triticum aestivum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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