Document Detail

Preparation and epitope mapping of a monoclonal antibody against Tri a Bd 27K, a major wheat allergen.
MedLine Citation:
PMID:  19734669     Owner:  NLM     Status:  MEDLINE    
We produced a monoclonal antibody (mAb) as a probe for detection of Tri a Bd 27K, a major wheat allergen. The mAb recognized the allergen purified from wheat flour, and the epitope on the allergen to the mAb was determined to be amino acid sequence (154)VPWVVVDGKPL(164) of Tri a Bd 27K. Of the amino acid residues on the epitope, the amino acid residues responsible for the binding to the mAb were found to be W156, D160, G161, and P163.
Nobuko Komiyama; Miki Hiemori; Masumi Kimoto; Makiko Suzuki; Hiromi Yamashita; Yoshitaka Takahashi; Hideaki Tsuji
Related Documents :
12139969 - Purification and characterization of a novel glutathione s-transferase from asaphis dic...
610279 - Amino acid sequence of the basic trypsin inhibitor from bovine splenic capsule. a new f...
21557089 - Degradation and detoxification of acid orange 52 by pseudomonas putida mt-2: a laborato...
23122069 - Evaluation and characterisation of citrullus colocynthis (l.) schrad seed oil: comparis...
23477719 - Importance of ortho proton donors in catalysis of hydrazone formation.
23518569 - Coproduction of acetic acid and electricity by application of microbial fuel cell techn...
1647439 - Okadaic acid inhibition of kcl cotransport. evidence that protein dephosphorylation is ...
5770619 - Feline attractant, cis,trans-nepetalactone: metabolism in the domestic cat.
11076579 - A beta-amino acid containing tripeptide from a pseudomonas-alteromonas bacterium associ...
Publication Detail:
Type:  Journal Article     Date:  2009-09-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  73     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-24     Completed Date:  2009-12-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2113-6     Citation Subset:  IM    
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal / chemistry,  immunology*
Antigens, Plant / immunology*
Base Sequence
Blotting, Western
DNA Primers
Electrophoresis, Polyacrylamide Gel
Epitope Mapping*
Polymerase Chain Reaction
Triticum / immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Plant; 0/DNA Primers; 0/Tri a Bd 27K allergen, Triticum aestivum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Metabolic Discrimination of Catharanthus roseus Calli According to Their Relative Locations Using (1...
Next Document:  Various Eicosanoids Modulate the Cellular and Humoral Immune Responses of the Beet Armyworm, Spodopt...