Document Detail

Preparation of Cationic Nanogels for Nucleic Acid Delivery.
MedLine Citation:
PMID:  22967138     Owner:  NLM     Status:  Publisher    
Cationic nanogels with site-selected functionality were designed for the delivery of nucleic acid payloads targeting numerous therapeutic applications. Functional cationic nanogels containing quaternized 2-(dimethylamino)ethyl methacrylate and crosslinkers with reducible disulfide moieties (qNG) were prepared by activators generated by electron transfer (AGET) atom transfer radical polymerization (ATRP) in an inverse miniemulsion. Polyplex formation between the qNG and nucleic acid exemplified by plasmid DNA (pDNA) and short interfering RNA (siRNA duplexes) were evaluated. The delivery of polyplexes was optimized for the delivery of pDNA and siRNA to the Drosophila Schneider 2 (S2) cell-line. The qNG/Nucleic acid (i.e. siRNA and pDNA) polyplexes were found to be superior to FuGene-HD in their ability to both transfect pDNA and siRNA under the standard test conditions.
Saadyah Eliezer Averick; Eduardo Paredes; Ainara Irastorza; Abiraman Srinivasan; Daniel John Siegwart; Andrew J D Magenau; Hong Y Cho; Arun R Shrivats; Eric Hsu; Jinku Kim; Shiguang Liu; Jeffrey O Hollinger; Subha Ranjan Das; Krzysztof Matyjaszewski
Related Documents :
20943378 - High temperature dilute acid pretreatment of coastal bermuda grass for enzymatic hydrol...
14208508 - Physiology of the sporulation process in clostridium botulinum. i. correlation of morph...
17909788 - Oleic acid delays and modulates the transition from respiratory to fermentative metabol...
540048 - Acetate is the preferred substrate for long-chain fatty acid synthesis in isolated spin...
2039548 - Properties of the mitochondrial membrane and carnitine palmitoyltransferase in the peri...
2816238 - Displacement of warfarin from human serum proteins by halothane anaesthesia.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-12
Journal Detail:
Title:  Biomacromolecules     Volume:  -     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  320-detector-row computed tomography arteriography using CO(2) gas to detect malignant liver tumors.
Next Document:  NDMA Formation by Chloramination of Ranitidine: Kinetics and Mechanism.