Document Detail


Preparation of Cationic Nanogels for Nucleic Acid Delivery.
MedLine Citation:
PMID:  22967138     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cationic nanogels with site-selected functionality were designed for the delivery of nucleic acid payloads targeting numerous therapeutic applications. Functional cationic nanogels containing quaternized 2-(dimethylamino)ethyl methacrylate and crosslinkers with reducible disulfide moieties (qNG) were prepared by activators generated by electron transfer (AGET) atom transfer radical polymerization (ATRP) in an inverse miniemulsion. Polyplex formation between the qNG and nucleic acid exemplified by plasmid DNA (pDNA) and short interfering RNA (siRNA duplexes) were evaluated. The delivery of polyplexes was optimized for the delivery of pDNA and siRNA to the Drosophila Schneider 2 (S2) cell-line. The qNG/Nucleic acid (i.e. siRNA and pDNA) polyplexes were found to be superior to FuGene-HD in their ability to both transfect pDNA and siRNA under the standard test conditions.
Authors:
Saadyah Eliezer Averick; Eduardo Paredes; Ainara Irastorza; Abiraman Srinivasan; Daniel John Siegwart; Andrew J D Magenau; Hong Y Cho; Arun R Shrivats; Eric Hsu; Jinku Kim; Shiguang Liu; Jeffrey O Hollinger; Subha Ranjan Das; Krzysztof Matyjaszewski
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-12
Journal Detail:
Title:  Biomacromolecules     Volume:  -     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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