Document Detail


Preparation of BMP-2 containing bovine serum albumin (BSA) nanoparticles stabilized by polymer coating.
MedLine Citation:
PMID:  18709447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The purpose of this study was to investigate the preparation process of bone morphogenetic protein-2 (BMP-2) containing bovine serum albumin (BSA) nanoparticles (NPs), and to assess the bioactivity of BMP-2 encapsulated in such NPs. METHODS: The NPs were prepared by a coacervation method, and the effects of process parameters on NP size and polydispersity were examined. Polymer coated NPs were characterized with respect to amount of adsorbed polymer, particle size and zeta potential. Using bone marrow stromal cells (BMSC), biocompatibility of the NPs was investigated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) Assay, and bioactivity of the encapsulated BMP-2 was investigated by alkaline phosphatase (ALP) induction and calcification. RESULTS: The size of NPs could be controlled in the 50-400 nm range by process parameters including BSA concentration, non-solvent:solvent ratio and pH value. After coating with cationic polymers, the particle size and zeta potential were significantly increased. MTT assay indicated no toxicity of both the uncoated and coated NPs on BMSC. Based on ALP induction and calcification, full retention of BMP-2 bioactivity was retained in the polymer-coated NPs. CONCLUSIONS: This study described a preparation procedure for BSA NPs with controllable particle size, and such polymer-coated BSA NPs are promising delivery agents for local and systemic administration of BMP-2 in bone regeneration.
Authors:
Guilin Wang; Kevin Siggers; Sufeng Zhang; Hongxing Jiang; Zhenghe Xu; Ronald F Zernicke; John Matyas; Hasan Uludağ
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-15
Journal Detail:
Title:  Pharmaceutical research     Volume:  25     ISSN:  0724-8741     ISO Abbreviation:  Pharm. Res.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-02     Completed Date:  2009-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2896-909     Citation Subset:  IM    
Affiliation:
Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, Alberta, Canada.
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MeSH Terms
Descriptor/Qualifier:
Acetone / chemistry
Animals
Bone Morphogenetic Protein 2 / chemistry*
Drug Delivery Systems
Drug Stability
Humans
Nanoparticles / chemistry*
Particle Size
Polyethyleneimine / chemistry
Polylysine / chemistry
Rats
Serum Albumin, Bovine / chemistry*
Chemical
Reg. No./Substance:
0/Bone Morphogenetic Protein 2; 0/Serum Albumin, Bovine; 25104-18-1/Polylysine; 67-64-1/Acetone; 9002-98-6/Polyethyleneimine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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