Document Detail

Preparation of [3H]-12-L-hydroxyeicosatetraenoic acid and its use in radioimmunoassay.
MedLine Citation:
PMID:  6774356     Owner:  NLM     Status:  MEDLINE    
An efficient method for the enzymic preparation of high specific radioactivity [3H]-12-L-hydroxyeicosatetraenoic acid (12-L-HETE) is described. This compound was used as a radiolabeled ligand in the radioimmunoassay (RIA) of 12-L-HETE. The accuracy of the RIA was checked by incubating [14C]arachidonic acid with platelet lipoxygenase, and measuring enzyme activity in the presence of the inhibitor, 1-phenyl-3-pyrazolidinone (phenidone). The amount of 12-L-HETE synthesized, determined by RIA, was found to be in agreement with that analyzed by radiochemical assay after thin layer plate chromatography.
J D Eskra; L Levine; T J Carty
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Prostaglandins and medicine     Volume:  5     ISSN:  0161-4630     ISO Abbreviation:  Prostaglandins Med     Publication Date:  1980 Sep 
Date Detail:
Created Date:  1980-11-25     Completed Date:  1980-11-25     Revised Date:  2001-11-02    
Medline Journal Info:
Nlm Unique ID:  7810330     Medline TA:  Prostaglandins Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  201-7     Citation Subset:  IM    
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MeSH Terms
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Arachidonic Acids / analysis,  chemical synthesis*
Lipoxygenase Inhibitors
Pyrazoles / pharmacology
Reg. No./Substance:
0/Arachidonic Acids; 0/Lipoxygenase Inhibitors; 0/Pyrazoles; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 92-43-3/phenidone

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