Document Detail


Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola.
MedLine Citation:
PMID:  23966667     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.
Authors:
Inge Van Bogaert; Guodong Zhang; Jun Yang; Jun-Yan Liu; Yonghao Ye; Wim Soetaert; Bruce D Hammock
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-08-21
Journal Detail:
Title:  Journal of lipid research     Volume:  54     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-10-10     Completed Date:  2014-05-14     Revised Date:  2014-11-04    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3215-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / metabolism
Ascomycota / enzymology*
Cytochrome P-450 Enzyme System / metabolism
Gene Knockout Techniques*
Hydroxyeicosatetraenoic Acids / biosynthesis*,  metabolism,  pharmacology
Hydroxylation
Hydroxysteroid Dehydrogenases / deficiency*,  genetics*,  metabolism
Mice
Neovascularization, Physiologic / drug effects
Oxidation-Reduction
Grant Support
ID/Acronym/Agency:
P42 ES004699/ES/NIEHS NIH HHS; P42 ES04699/ES/NIEHS NIH HHS; R01 ES002710/ES/NIEHS NIH HHS; R01 ES02710/ES/NIEHS NIH HHS; U24 DK097154/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Hydroxyeicosatetraenoic Acids; 27YG812J1I/Arachidonic Acid; 79551-85-2/19-hydroxy-5,8,11,14-eicosatetraenoic acid; 79551-86-3/20-hydroxy-5,8,11,14-eicosatetraenoic acid; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.1.-/Hydroxysteroid Dehydrogenases; EC 1.1.1.209/3(17)-hydroxysteroid dehydrogenase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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