Document Detail


Preoperative diagnosis of suspicious parathyroid adenomas by RT-PCR using mRNA extracted from leftover cells in a needle used for ultrasonically guided fine needle aspiration cytology.
MedLine Citation:
PMID:  12585024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine the usefulness of reverse transcriptase-polymerase chain reaction (RT-PCR) detection of parathormone (PTH) gene mRNA in needle aspirates to confirm the parathyroid nature of suspicious cervical lesions in patients with hyperparathyroidism. STUDY DESIGN: Ultrasound-guided fine needle aspiration cytology (FNAC) was performed on 12 patients with suspected parathyroid adenomas. The aspirates were subjected to cytologic and chromogranin A examination. Messenger RNA was isolated from left-over cells within the needle, and RT-PCR was used to amplify mRNA encoding PTH. RESULTS: The 12 aspirates were positive by PTH RT-PCR, and molecular diagnosis was confirmed by histologic examination. The lower sensitivity of cytologic and immunocytochemical methods was demonstrated, respectively, by 67% and 50% of positive results. Sensitivity assays demonstrated that with our system, PTH RT-PCR products are obtained from as few as 10 pg of parathyroid mRNA. CONCLUSION: The present study showed that RT-PCR-based analysis of PTH gene transcripts in aspirates, obtained by US-guided FNAC of cervical lesions suspicious for parathyroid adenoma, is a feasible, sensitive and specific method for preoperative diagnosis of parathyroid adenomas.
Authors:
Branca M Cavaco; Fernando Torrinha; Evelina Mendonça; Sónia Pratas; José Boavida; Luís G Sobrinho; Valeriano Leite
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta cytologica     Volume:  47     ISSN:  0001-5547     ISO Abbreviation:  Acta Cytol.     Publication Date:    2003 Jan-Feb
Date Detail:
Created Date:  2003-02-14     Completed Date:  2003-03-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370307     Medline TA:  Acta Cytol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5-12     Citation Subset:  IM    
Affiliation:
Center for the Investigation of Molecular Pathobiology, Departments of Imagiology and Endocrinology, Cytology Laboratory, Francisco Gentil Portuguese Oncology Institute, Lisbon, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Adenoma / diagnosis*,  pathology
Adult
Aged
Biopsy, Needle / methods*
Chromogranin A
Chromogranins / metabolism
Female
Humans
Male
Middle Aged
Molecular Diagnostic Techniques*
Parathyroid Hormone / genetics*
Parathyroid Neoplasms / diagnosis*,  pathology,  ultrasonography
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction*
Sensitivity and Specificity
Ultrasonography
Chemical
Reg. No./Substance:
0/Chromogranin A; 0/Chromogranins; 0/Parathyroid Hormone; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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