Document Detail


Preoperative depletion of C3 improves the survival of guinea pig-to-rat cardiac xenograft recipients.
MedLine Citation:
PMID:  9100173     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat strains with congenitally reduced total hemolytic complement activity do not reject cardiac xenografts hyperacutely. Prolongation of graft survival in the guinea pig-to-C6-deficient PVG rat donor/recipient combination has been observed. However, experience with this model has been complicated by a high postoperative mortality from respiratory distress. The authors hypothesized that placement of the xenograft resulted in local activation of complement, which contributed to remote pulmonary injury leading to respiratory dysfunction. To test this hypothesis, an attempt was made to reduce early complement component activation with the use of an antibody to rat C3 in C6-deficient PVG recipients. Six of eight untreated C6-deficient PVG recipients died in the immediate postoperative period with vigorously beating heart grafts, whereas only 2 of 14 C6-deficient recipients pretreated with anti-rat C3 antibody died within 24 h postoperatively. Although pretreatment with anti-C3 antibody improved survival of recipients, the duration of cardiac xenograft survival was similar whether the recipients were pretreated or not. The use of anti-C3 antibody in C6-deficient rats is a valid approach to studying xenotransplantation in the absence of hyperacute rejection and has an additional advantage in that it does not require the use of expensive reagents such as cobra venom factor.
Authors:
C H Suh; M K Oaks; N N Dong; J G Pellegrini; D C Kress; A J Tector
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of investigative surgery : the official journal of the Academy of Surgical Research     Volume:  10     ISSN:  0894-1939     ISO Abbreviation:  J Invest Surg     Publication Date:    1997 Jan-Apr
Date Detail:
Created Date:  1997-06-09     Completed Date:  1997-06-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809255     Medline TA:  J Invest Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  37-40     Citation Subset:  IM    
Affiliation:
Transplant Research Laboratory, St. Luke's Medical Center, Milwaukee, Wisconsin 63215, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / pharmacology
Antibody Specificity
Binding, Competitive / immunology
Complement C3 / immunology*,  metabolism
Complement C6 / deficiency
Female
Graft Rejection / immunology,  prevention & control*
Guinea Pigs
Heart Transplantation / immunology*,  mortality
Lung / blood supply,  pathology
Rats
Rats, Inbred Strains
Reperfusion Injury / physiopathology
Survival Analysis
Transplantation, Heterologous / immunology*,  mortality
Chemical
Reg. No./Substance:
0/Antibodies; 0/Complement C3; 0/Complement C6

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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