| Prenatal urinary matrix metalloproteinase profiling as a potential diagnostic tool in fetal obstructive uropathy. | |
| | |
MedLine Citation:
|
PMID: 20105582 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND/PURPOSE: The diagnostic evaluation, patient stratification, and prenatal counseling for congenital obstructive uropathy remain sub-optimal. Matrix metalloproteinase (MMP) expression profiles are emerging as a valuable diagnostic tool in assorted disease processes. We sought to determine whether congenital obstructive uropathy impacts MMP expression in fetal urine. METHODS: Fetal lambs (n = 25) were divided in two groups: group I (n = 12) underwent a sham operation and group II (n = 13) underwent creation of a complete urinary tract obstruction. Gelatin zymography panels for 4 MMP species were performed on fetal urine in both groups at comparable times post-operatively. Statistical analysis was by the Fisher's exact test (P < .05). RESULTS: Overall fetal survival was 80% (20/25). A variety of significant differences in MMP expression between the two groups were identified. The following profiles were present only in obstructed animals: any MMP other than MMP-2 (P = .029), including any MMP other than 63 kDa and 65 kDa (P = .009); 2 or more MMPs excluding MMP-2s (0.029); and 3 or more MMPs (P = .029). CONCLUSIONS: Limited matrix metalloproteinase expression is present in the urine of normal ovine fetuses. Fetal obstructive uropathy impacts urinary MMP expression in various distinguishable patterns. Prenatal urinary MMP profiling may become a practical and valuable diagnostic tool in the evaluation of congenital obstructive uropathy. |
| | |
Authors:
|
Grace A Nicksa; David C Yu; Adam S Curatolo; Brendan L McNeish; Carol E Barnewolt; Clarissa Valim; Terry L Buchmiller; Marsha A Moses; Dario O Fauza |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Journal of pediatric surgery Volume: 45 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2010-01-28 Completed Date: 2010-03-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
|
Languages: eng Pagination: 70-3 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Surgery, Children's Hospital Boston & Harvard Medical School, Boston, MA 02115, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Female Fetus / metabolism Matrix Metalloproteinase 2 / urine Matrix Metalloproteinase 9 / urine Matrix Metalloproteinases / urine* Matrix Metalloproteinases, Secreted / urine Pregnancy Sheep Tissue Inhibitor of Metalloproteinase-1 / urine Ultrasonography, Prenatal Urologic Diseases / congenital*, enzymology, urine* |
| Chemical | |
Reg. No./Substance:
|
0/Tissue Inhibitor of Metalloproteinase-1; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.-/Matrix Metalloproteinases, Secreted; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In utero repair of gastroschisis in the sheep (Ovis aries) model.
Next Document: A prospective trial for laparoscopic adjustable gastric banding in morbidly obese adolescents: an in...