Document Detail

Prenatal sexing and sex determination in infants with ambiguous genitalia by polymerase chain reaction.
MedLine Citation:
PMID:  10464660     Owner:  NLM     Status:  MEDLINE    
Sex determination in both fetuses and infants with ambiguous external genitalia usually necessitates time-consuming and costly karyotyping. We propose a simple, rapid, and reliable method of prenatal and postnatal sex determination by means of the PCR, a technique currently used to identify gender for forensic purposes. DNA was extracted from 20 samples of whole blood from infants with ambiguous genitalia and from five samples of amniotic fluid. Three markers were amplified from each specimen: a Y chromosome alphoid repeat sequence and two homologous genes, amelogenin (AME) and zinc finger protein (ZFP). All three were detected in under 10 hr. A comparison of the results obtained with those of cytogenetic analysis of the same samples showed a perfect sex match, demonstrating that this PCR technique provides an accurate means of determining gender.
L Caenazzo; E Ponzano; N A Greggio; P Cortivo
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Genetic testing     Volume:  1     ISSN:  1090-6576     ISO Abbreviation:  Genet. Test.     Publication Date:    1997-1998
Date Detail:
Created Date:  1999-10-12     Completed Date:  1999-10-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9802546     Medline TA:  Genet Test     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  289-91     Citation Subset:  IM    
Institute of Legal Medicine, University of Padua, Italy.
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MeSH Terms
Dental Enamel Proteins / genetics
Genitalia / abnormalities*
Polymerase Chain Reaction*
Prenatal Diagnosis*
Repetitive Sequences, Nucleic Acid
Sex Determination (Analysis)*
Y Chromosome / genetics
Zinc Fingers
Reg. No./Substance:
0/Amelogenin; 0/Dental Enamel Proteins

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