Document Detail

Prenatal protein restriction does not affect the proliferation and differentiation of rat preadipocytes.
MedLine Citation:
PMID:  15173417     Owner:  NLM     Status:  MEDLINE    
Poor development in utero may favor the development of obesity in adulthood. Animal studies showed that embryo manipulation in vitro or nutritional insults during the embryonic and fetal stages of development may lead to obesity in adult life. We studied the in vitro proliferation and differentiation of adipocytes to investigate whether early protein restriction may program cell growth and development. In a series of experiments, 2 different low-protein diet protocols were compared. In both cases, pregnant rats were fed a diet with a high (18-20%) or low (8-9%) protein content during gestation and/or lactation. Preadipocytes were isolated from the fetuses, neonates, and weanling offspring. Moderate protein restriction, imposed during either gestation and/or lactation, did not affect the capacity of preadipose cells to divide or store fat. Because previous studies showed that early protein restriction alters the metabolism of sulfur amino acids, we also investigated the effects of methionine, taurine, and homocysteine on proliferation and differentiation of preadipocytes. The supplementation of the diet with methionine or the addition of homocysteine and taurine to the culture media did not influence the development of preadipocytes. We obtained no evidence for the direct reprogramming of the precursor or stem cells and suggest that the subsequent alteration in fat accretion may therefore reflect a change in the neuroendocrine environment.
Florence Bieswal; Susan M Hay; Caroline McKinnon; Brigitte Reusens; Marie Cuignet; William D Rees; Claude Remacle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  134     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-02     Completed Date:  2004-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1493-9     Citation Subset:  IM    
Laboratory of Cell Biology, 1348 Louvain-la-Neuve, Belgium.
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MeSH Terms
Adipocytes / cytology*
Animals, Newborn / growth & development
Blood Vessels / cytology,  embryology
Cell Differentiation
Cell Division
Cells, Cultured
Culture Media
Diet, Protein-Restricted*
Epididymis / cytology
Fetus / cytology
Homocysteine / administration & dosage
Kidney / cytology
Methionine / administration & dosage
Pregnancy, Animal*
Rats, Inbred Strains
Stem Cells / cytology*
Stromal Cells / cytology
Subcutaneous Tissue / blood supply,  embryology
Taurine / administration & dosage
Reg. No./Substance:
0/Culture Media; 107-35-7/Taurine; 454-28-4/Homocysteine; 63-68-3/Methionine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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