Document Detail

Prenatal diagnosis of trisomy 21 with fetal cells in maternal blood using comparative genomic hybridization.
MedLine Citation:
PMID:  16354990     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: This study was undertaken to determine the clinical use of comparative genomic hybridization (CGH) for detection of fetal trisomy 21 from fetal ceIls (nucleated red blood cells; nRBCs) isolated from maternal peripheral venous blood. METHODS: Maternal peripheral venous blood samples were collected in sterile tubes containing heparin. After triple density gradient centrifugation, magnetic activated cell sorting using CD45 and CD71 was used to isolate the fetal nRBCs. Fetal nRBCs were successfully isolated from maternal peripheral blood in all cases. After laser-microdissecting fetal nRBCs, degenerate oligonucleotide-primed polymerase chain reaction, and nick translation, DNA size was suitable for hybridization. RESULTS: By CGH analysis, we diagnosed one normal male, one normal female, and one trisomy 21 male fetus. These results were confirmed by amniocentesis. CONCLUSIONS: Prenatal diagnosis from fetal cells in maternal peripheral blood by CGH shows clinical promise as an alternative or as a supplement to fluorescence in situ hybridization with chromosome-specific probes but further studies are warranted.
Young Ho Yang; Eun Suk Yang; Ja Young Kwon; In Kyu Kim; Yong Won Park
Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Fetal diagnosis and therapy     Volume:  21     ISSN:  1015-3837     ISO Abbreviation:  Fetal. Diagn. Ther.     Publication Date:  2006  
Date Detail:
Created Date:  2005-12-15     Completed Date:  2006-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107463     Medline TA:  Fetal Diagn Ther     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  125-33     Citation Subset:  IM    
Copyright Information:
(c) 2006 S. Karger AG, Basel
Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea.
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MeSH Terms
DNA / analysis
Down Syndrome / diagnosis*,  embryology,  genetics
Erythrocytes / cytology*
Fetus / cytology*
Nucleic Acid Hybridization / methods*
Placental Circulation
Prenatal Diagnosis*
Reg. No./Substance:

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