Document Detail


Prenatal diagnosis of neonatal alloimmune thrombocytopenia using allele-specific oligonucleotide probes.
MedLine Citation:
PMID:  1932744     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The prediction of neonatal alloimmune thrombocytopenia (NATP) in affected families has, in the past, been based on information about gene frequencies of the antigen systems involved, parental phenotyping, and fetal platelet counts. We explored the feasibility of allele-specific oligonucleotide probe typing for PIA antigens to determine the risk of second or subsequent fetuses in families where one infant had a diagnosis of anti-PIA1-mediated NATP. A total of eight families at risk for delivering an affected fetus were studied with both serologic and oligonucleotide typing. The correlation between serologic and oligonucleotide PIA types was 100%. Similarly, in an additional eight families not at risk for PIA1-mediated NATP, serologic and oligonucleotide typing maintained a perfect correlation. DNA isolated from fetal leukocytes as well as fetal amniocytes was successfully typed using this technology. Oligonucleotide-based typing of fetuses at risk for NATP whose fathers are heterozygous for the PIA antigens allows early recognition of affected fetuses so that prenatal therapy of mothers can be instituted if necessary. When fetuses are found to be unaffected, invasive, and/or expensive, prenatal interventions can be avoided.
Authors:
J G McFarland; R H Aster; J B Bussel; J G Gianopoulos; R S Derbes; P J Newman
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  78     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1991-12-02     Completed Date:  1991-12-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2276-82     Citation Subset:  AIM; IM    
Affiliation:
Blood Center of Southeastern Wisconsin, Milwaukee 53233.
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MeSH Terms
Descriptor/Qualifier:
Adult
Alleles
Antibodies / blood
Antigens, Human Platelet / analysis*,  genetics,  immunology
Base Sequence
Blood Platelets / immunology*
Female
Humans
Immunoglobulin Allotypes / immunology*
Infant, Newborn
Molecular Sequence Data
Oligonucleotide Probes*
Phenotype
Pregnancy
Prenatal Diagnosis / methods*
Thrombocytopenia / diagnosis,  immunology*,  therapy
Grant Support
ID/Acronym/Agency:
HL-13629/HL/NHLBI NIH HHS; HL-44612/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/1a alloantigen, human; 0/Antibodies; 0/Antigens, Human Platelet; 0/Immunoglobulin Allotypes; 0/Oligonucleotide Probes

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