| Prenatal development of murine gonads with special reference to germ cell differentiation: a morphological and immunohistochemical study. | |
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MedLine Citation:
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PMID: 17683469 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The prenatal differentiation of male and female gonads of the mouse was investigated both morphologically and immunohistochemically. Sexual dimorphism could be detected as early as 12 days post-coitum (dpc) by the appearance of the primary elements of the tunica albuginea and positive immunoreactivity for anti-Muellerian hormone in the Sertoli cells of the male gonad. Male germ cells passed two waves of mitotic activity, a first wave between 12 and 14 dpc, which is followed by apoptosis of the old germ cell generation, and a second wave between 17 and 20 dpc. Oct-4 was expressed as a juxtanuclear ring in the cytoplasm of germ cells up to 17 dpc. Subsequently, it was down-regulated and completely disappeared in 20 dpc full-term fetuses. By contrast, M2A antigen revealed only a weak immunoreaction in some germ cells of 14 dpc gonads, but exhibited strong signals in all germ cells of 20 dpc full-term fetuses. Therefore, we postulate that, in the mouse, prenatal germ cells represent two populations: the first is immunopositive for Oct-4 and disappeared in full-term fetuses, whereas the second appeared in 14 dpc and is immunopositive for M2A antigen. |
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Authors:
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A E Zayed; M M Abd-Elnaeim; S Kh Abd-Elghaffar; A Hild; R Brehm; K Steger |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Andrologia Volume: 39 ISSN: 0303-4569 ISO Abbreviation: Andrologia Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-08-08 Completed Date: 2007-08-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0423506 Medline TA: Andrologia Country: Germany |
Other Details:
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Languages: eng Pagination: 93-100 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Histology, Faculty of Veterinary Medicine, University of Assiut, Assiut, Egypt. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Mullerian Hormone Apoptosis Cell Differentiation / physiology* Female Germ Cells / cytology Glycoproteins / metabolism Humans Immunohistochemistry Male Mice Octamer Transcription Factor-3 / metabolism Ovary / embryology*, metabolism Sex Differentiation / physiology Testicular Hormones / metabolism Testis / embryology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Octamer Transcription Factor-3; 0/Testicular Hormones; 80497-65-0/Anti-Mullerian Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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