Document Detail

Prenatal development of murine gonads with special reference to germ cell differentiation: a morphological and immunohistochemical study.
MedLine Citation:
PMID:  17683469     Owner:  NLM     Status:  MEDLINE    
The prenatal differentiation of male and female gonads of the mouse was investigated both morphologically and immunohistochemically. Sexual dimorphism could be detected as early as 12 days post-coitum (dpc) by the appearance of the primary elements of the tunica albuginea and positive immunoreactivity for anti-Muellerian hormone in the Sertoli cells of the male gonad. Male germ cells passed two waves of mitotic activity, a first wave between 12 and 14 dpc, which is followed by apoptosis of the old germ cell generation, and a second wave between 17 and 20 dpc. Oct-4 was expressed as a juxtanuclear ring in the cytoplasm of germ cells up to 17 dpc. Subsequently, it was down-regulated and completely disappeared in 20 dpc full-term fetuses. By contrast, M2A antigen revealed only a weak immunoreaction in some germ cells of 14 dpc gonads, but exhibited strong signals in all germ cells of 20 dpc full-term fetuses. Therefore, we postulate that, in the mouse, prenatal germ cells represent two populations: the first is immunopositive for Oct-4 and disappeared in full-term fetuses, whereas the second appeared in 14 dpc and is immunopositive for M2A antigen.
A E Zayed; M M Abd-Elnaeim; S Kh Abd-Elghaffar; A Hild; R Brehm; K Steger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Andrologia     Volume:  39     ISSN:  0303-4569     ISO Abbreviation:  Andrologia     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-08-08     Completed Date:  2007-08-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  93-100     Citation Subset:  IM    
Department of Anatomy and Histology, Faculty of Veterinary Medicine, University of Assiut, Assiut, Egypt.
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MeSH Terms
Anti-Mullerian Hormone
Cell Differentiation / physiology*
Germ Cells / cytology
Glycoproteins / metabolism
Octamer Transcription Factor-3 / metabolism
Ovary / embryology*,  metabolism
Sex Differentiation / physiology
Testicular Hormones / metabolism
Testis / embryology*,  metabolism
Reg. No./Substance:
0/Glycoproteins; 0/Octamer Transcription Factor-3; 0/Testicular Hormones; 80497-65-0/Anti-Mullerian Hormone

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