| Prenatal alcohol exposure triggers ceramide-induced apoptosis in neural crest-derived tissues concurrent with defective cranial development. | |
| | |
MedLine Citation:
|
PMID: 21364652 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Fetal alcohol syndrome (FAS) is caused by maternal alcohol consumption during pregnancy. The reason why specific embryonic tissues are sensitive toward ethanol is not understood. We found that in neural crest-derived cell (NCC) cultures from the first branchial arch of E10 mouse embryos, incubation with ethanol increases the number of apoptotic cells by fivefold. Apoptotic cells stain intensely for ceramide, suggesting that ceramide-induced apoptosis mediates ethanol damage to NCCs. Apoptosis is reduced by incubation with CDP-choline (citicoline), a precursor for the conversion of ceramide to sphingomyelin. Consistent with NCC cultures, ethanol intubation of pregnant mice results in ceramide elevation and increased apoptosis of NCCs in vivo. Ethanol also increases the protein level of prostate apoptosis response 4 (PAR-4), a sensitizer to ceramide-induced apoptosis. Prenatal ethanol exposure is concurrent with malformation of parietal bones in 20% of embryos at day E18. Meninges, a tissue complex derived from NCCs, is disrupted and generates reduced levels of TGF-β1, a growth factor critical for bone and brain development. Ethanol-induced apoptosis of NCCs leading to defects in the meninges may explain the simultaneous presence of cranial bone malformation and cognitive retardation in FAS. In addition, our data suggest that treatment with CDP-choline may alleviate the tissue damage caused by alcohol. |
| | |
Authors:
|
G Wang; E Bieberich |
Publication Detail:
|
Type: Journal Article Date: 2010-05-27 |
Journal Detail:
|
Title: Cell death & disease Volume: 1 ISSN: 2041-4889 ISO Abbreviation: Cell Death Dis Publication Date: 2010 May |
Date Detail:
|
Created Date: 2011-03-02 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101524092 Medline TA: Cell Death Dis Country: England |
Other Details:
|
Languages: eng Pagination: e46 Citation Subset: IM |
Affiliation:
|
Institute of Molecular Medicine and Genetics, School of Medicine, Medical College of Georgia, 1120 15th Street Room CA-4012, Augusta, GA 30912, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protein kinase C? is required for cardiomyocyte survival and cardiac remodeling.
Next Document: Autism spectrum disorder is related to endoplasmic reticulum stress induced by mutations in the syna...