| Prenatal NAP+SAL prevents developmental delay in a mouse model of Down syndrome through effects on N-methyl-D-aspartic acid and gamma-aminobutyric acid receptors. | |
| | |
MedLine Citation:
|
PMID: 19327737 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: Down syndrome (DS) affects 1/800 infants. Prenatal NAPVSIPQ (NAP) and SALLRSIPA (SAL) (NAP+SAL) prevent developmental delay in Ts65Dn mice, a mouse model of DS. We investigated whether this finding involves N-methyl-D-aspartic acid and gamma-aminobutyric acid (GABA) receptor subunits. STUDY DESIGN: Pregnant Ts65Dn mice were treated with placebo or NAP+SAL on gestational days 8-12. After developmental delay prevention was shown, 4 trisomic (Ts), 4 control, and 3 Ts+NAP+SAL adult offspring brains (from 3 litters) were collected. Calibrator-normalized real-time polymerase chain reaction was performed using primers for N-methyl-D-aspartic acid subunits NR2A and NR2B, and for GABA subunits GABA(A)alpha5 and GABA(A)beta3 with glyceraldehyde-3-phosphate dehydrogenase standardization. Statistics included analysis of variance and Fisher PLSD with P < .05 as significant. RESULTS: NR2A, NR2B, and GABA(A)beta3 levels were decreased in Ts vs control (all P < .05). Prenatal NAP+SAL increased NR2A, NR2B, and GABA(A)beta3 to levels similar to control (all P < .05). A significant difference in GABA(A)alpha5 levels was not found. CONCLUSION: Prenatal NAP+SAL increases NR2A, NR2B, and GABA(A)beta3 expression in adult DS mice to levels similar to controls. This may explain how NAP+SAL improve developmental milestone achievement. |
| | |
Authors:
|
Joy Vink; Maddelena Incerti; Laura Toso; Robin Roberson; Daniel Abebe; Catherine Y Spong |
Related Documents
:
|
12742517 - Potentiation of metabotropic gabab receptors by l-amino acids and dipeptides in rat neo... 3000377 - Neurotoxic insecticides inhibit gaba-dependent chloride uptake by mouse brain vesicles. 12063087 - Central effects of 1,4-butanediol are mediated by gaba(b) receptors via its conversion ... 15120317 - Design, synthesis and anticonvulsive activity of analogs of gamma-vinyl gaba. 15212477 - Comparative study of the composition of melanoidins from glucose and maltose. 4898997 - Sodium-stimulated transport of glutamate in escherichia coli. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Intramural Date: 2009-03-26 |
Journal Detail:
|
Title: American journal of obstetrics and gynecology Volume: 200 ISSN: 1097-6868 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 2009 May |
Date Detail:
|
Created Date: 2009-04-20 Completed Date: 2009-05-18 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: United States |
Other Details:
|
Languages: eng Pagination: 524.e1-4 Citation Subset: AIM; IM |
Affiliation:
|
Unit on Perinatal and Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Brain Diseases / drug therapy*, genetics, prevention & control* Central Nervous System / drug effects, embryology, growth & development Disease Models, Animal Down Syndrome / complications*, genetics Female Mental Retardation / drug therapy, prevention & control Mice Mice, Mutant Strains Nerve Tissue Proteins / pharmacology* Oligopeptides / pharmacology* Peptide Fragments / pharmacology* Pregnancy Prenatal Exposure Delayed Effects Receptors, GABA-A / genetics, metabolism Receptors, N-Methyl-D-Aspartate / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Gabra5 protein, mouse; 0/NAPVSIPQ peptide; 0/NR2A NMDA receptor; 0/NR2B NMDA receptor; 0/Nerve Tissue Proteins; 0/Oligopeptides; 0/Peptide Fragments; 0/Receptors, GABA-A; 0/Receptors, N-Methyl-D-Aspartate; 0/SALLRSIPA; 0/beta3 subunit, GABA-A receptor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: TMEM126A, encoding a mitochondrial protein, is mutated in autosomal-recessive nonsyndromic optic atr...
Next Document: Recovery of corneal hysteresis after reduction of intraocular pressure in chronic primary angle-clos...