| Prenatal Diagnosis of LAD-I on Cord Blood by Flowcytometry. | |
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MedLine Citation:
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PMID: 22477041 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVES: To optimize a simple flowcytometric technique for Prenatal diagnosis (PND) for Leukocyte adhesions defect (LAD-I) on cordocentesis sample at 18 wk gestation. METHODS: Normal reference ranges for expression of CD18/CD11-integrins in neutrophils and lymphocytes at 18 wk of gestation were established by flowcytometry. PND for LAD-I was then performed on the cordocentesis samples in three 'at risk' pregnancies after ruling out maternal contamination. RESULTS: CD18 and CD11a expression on fetal lymphocytes were found to be the most useful parameters for PND of LAD-I. All the three fetuses tested showed normal expression of CD18/CD11-integrins and thus were unaffected. This was confirmed by testing the cord blood (CB) samples after delivery and normal growth and absence of serious infections on follow-up. CONCLUSIONS: Flowcytometry offers a rapid and sensitive technique for PND of LAD-I in the absence of facilities for molecular diagnosis. Obstetricians, even in developing countries with modest facilities, can offer considerable relief for the families. |
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Authors:
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Manisha Rajan Madkaikar; Maya Gupta; Meghana Rao; Kanjaksha Ghosh |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-4 |
Journal Detail:
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Title: Indian journal of pediatrics Volume: - ISSN: 0973-7693 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417442 Medline TA: Indian J Pediatr Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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National Institute of Immunohematlogy, Pediatric Immunology and Leukocyte Biology, 13th Flr, KEM Hospital, Parel, Mumbai, Maharashtra, 400012, India, madkaikarm@icmr.org.in. |
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