Document Detail

Premalignancy in vitro: progression of an initially benign epithelial cell line to malignancy.
MedLine Citation:
PMID:  540098     Owner:  NLM     Status:  MEDLINE    
An epithelial cell line, NMuLi, derived from normal mouse liver, produced a variety of tumours when inoculated s.c. into newborn mice. The inoculation of early-passage cells resulted in cystadenomas and proliferating cysts, while adenocarcinomas and carcinosarcomas were produced from cells that had been extensively subcultured. To determine the mechanism involved in the progression to malignancy, single-cell clones were isolated from early- and late-passage NMuLi cells. When inoculated into mice, early-passage clones were similar to the original population in that they initially formed benign cysts but produced increasingly aberrant tumours after extensive subculture. Inoculation of the late-passage clones resulted in only malignant tumours. These observations suggest that passage in culture induced an initially benign epithelial cell population to progress toward malignancy; thus early passage NMuLi cells represent a good in vitro model of the premalignant state.
L W Anderson; H S Smith
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of experimental pathology     Volume:  60     ISSN:  0007-1021     ISO Abbreviation:  Br J Exp Pathol     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-06-25     Completed Date:  1980-06-25     Revised Date:  2012-05-07    
Medline Journal Info:
Nlm Unique ID:  0372543     Medline TA:  Br J Exp Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  575-81     Citation Subset:  IM    
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MeSH Terms
Adenocarcinoma / pathology
Carcinosarcoma / pathology
Cell Line
Cell Transformation, Neoplastic / pathology*
Clone Cells / pathology
Cystadenoma / pathology
Cysts / pathology
Epithelial Cells
Liver / cytology
Neoplasms, Experimental / pathology*
Precancerous Conditions / pathology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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