| Preliminary results of fetal Rhc examination in plasma of pregnant women with anti-c. | |
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MedLine Citation:
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PMID: 18382999 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Anti-Rhc antibodies may be the reason for the hemolytic disease of the newborn, therefore, noninvasive Rhc determination is important for pregnancy monitoring. For this purpose, we decided to introduce real-time polymerase chain reaction (PCR) method. METHODS: Blood from 200 donors, plasma and whole-blood from 11 Rhc-negative mothers, as well as blood from fathers and newborns were examined. Rhc sensitivity and specificity were first determined by real-time PCR using genomic DNA from donors. The same Rhc genotyping method was used for fetal Rhc detection in maternal plasma. To confirm the fetal Rhc-negative result, plasma was tested with a panel of biallelic insertion/deletion polymorphisms for the presence of fetal DNA. RESULTS: The c allele assay showed full specificity. The mean Ct value for one copy of c allele diluted in C-negative DNA was determined from extrapolating the correlation curve as 39.9. Full concordance was observed between the fetal Rhc genotypes from maternal plasma and the newborn phenotypes. CONCLUSIONS: Preliminary results show that it is possible to examine fetal c allele of RHCE gene in the plasma of pregnant women with anti-c by means of a noninvasive method. The diagnostic accuracy of the procedure, however, has yet to be confirmed in a larger group. |
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Authors:
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A Orzińska; K Guz; E Brojer; B Zupańska |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Prenatal diagnosis Volume: 28 ISSN: 0197-3851 ISO Abbreviation: Prenat. Diagn. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-04-03 Completed Date: 2008-08-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8106540 Medline TA: Prenat Diagn Country: England |
Other Details:
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Languages: eng Pagination: 335-7 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2008 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Immunohematology and Immunology of Transfusion Medicine, Institute of Hematology and Transfusion Medicine, 14 Gandhi Street, 02 776 Warsaw, Poland. orzinska@ihit.waw.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Feasibility Studies Female Fetus / metabolism* Genotype Gestational Age Humans Infant, Newborn Isoantibodies / blood* Male Maternal-Fetal Exchange / genetics, physiology Mothers* Pregnancy Prenatal Diagnosis / methods Rh-Hr Blood-Group System / blood*, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Isoantibodies; 0/RHCE protein, human; 0/Rh-Hr Blood-Group System; 0/anti-c antibody |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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