Document Detail

Preliminary results of fetal Rhc examination in plasma of pregnant women with anti-c.
MedLine Citation:
PMID:  18382999     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Anti-Rhc antibodies may be the reason for the hemolytic disease of the newborn, therefore, noninvasive Rhc determination is important for pregnancy monitoring. For this purpose, we decided to introduce real-time polymerase chain reaction (PCR) method. METHODS: Blood from 200 donors, plasma and whole-blood from 11 Rhc-negative mothers, as well as blood from fathers and newborns were examined. Rhc sensitivity and specificity were first determined by real-time PCR using genomic DNA from donors. The same Rhc genotyping method was used for fetal Rhc detection in maternal plasma. To confirm the fetal Rhc-negative result, plasma was tested with a panel of biallelic insertion/deletion polymorphisms for the presence of fetal DNA. RESULTS: The c allele assay showed full specificity. The mean Ct value for one copy of c allele diluted in C-negative DNA was determined from extrapolating the correlation curve as 39.9. Full concordance was observed between the fetal Rhc genotypes from maternal plasma and the newborn phenotypes. CONCLUSIONS: Preliminary results show that it is possible to examine fetal c allele of RHCE gene in the plasma of pregnant women with anti-c by means of a noninvasive method. The diagnostic accuracy of the procedure, however, has yet to be confirmed in a larger group.
A Orzińska; K Guz; E Brojer; B Zupańska
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  28     ISSN:  0197-3851     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-03     Completed Date:  2008-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  335-7     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2008 John Wiley & Sons, Ltd.
Department of Immunohematology and Immunology of Transfusion Medicine, Institute of Hematology and Transfusion Medicine, 14 Gandhi Street, 02 776 Warsaw, Poland.
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MeSH Terms
Feasibility Studies
Fetus / metabolism*
Gestational Age
Infant, Newborn
Isoantibodies / blood*
Maternal-Fetal Exchange / genetics,  physiology
Prenatal Diagnosis / methods
Rh-Hr Blood-Group System / blood*,  genetics,  metabolism*
Reg. No./Substance:
0/Isoantibodies; 0/RHCE protein, human; 0/Rh-Hr Blood-Group System; 0/anti-c antibody

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