| Preliminary investigations on a new disposable potentiometric biosensor for uric acid. | |
| | |
MedLine Citation:
|
PMID: 16830944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this paper, uricase, catalase, and electron mediator were coimmobilized on the surface of the tin oxide (SnO2)/indium tin oxide (ITO) glass, to develop a disposable potentiometric uric acid biosensor. The SnO2/ITO glass was employed as a pH sensor, fabricated by sputtering SnO2 thin films on the ITO glass. 3-Glycidyloxypropyltrimethoxysilane (GPTS) was utilized to immobilize uricase, catalase and the electron mediator (ferrocenecarboxylic acid, FcA) on the sensing window. The experimental results reveal that the optimal weight ratio of uricase, FcA to catalase (CAT) is 4:1:2. The sensor responds linearly between 2 mg/dl and 7 mg/dl at pH 7.5, in 20 mM of test solution, with a correlation coefficient of 0.99213. Accordingly, no significant interference was observed when interfering substances, glucose, urea and ascorbic acid, were added to the uric acid solution. Moreover, the recorded voltage was relatively constant during the first 28 days of measurement. Consequently, a potentiometric uric acid biosensor was realized with the advantages of low cost and simple fabrication. |
| | |
Authors:
|
Cheng Wei Liao; Jung Chuan Chou; Tai Ping Sun; Shen Kan Hsiung; Jui Hsiang Hsieh |
Related Documents
:
|
18390574 - Carbon fibre composites: integrated electrochemical sensors for wound management. 12510264 - Permselective monolayer membrane based on two-dimensional cross-linked polysiloxane lb ... 4096374 - Acetaldehyde: its "disappearance" from human blood. 7125574 - Evaluation of a quantitative solid phase reagent system for determination of blood anal... 4868364 - Lipids of salmonella typhimurium and escherichia coli: structure and metabolism. 3965024 - Polyunsaturated fatty acids and diabetic retinopathy. |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: IEEE transactions on bio-medical engineering Volume: 53 ISSN: 0018-9294 ISO Abbreviation: IEEE Trans Biomed Eng Publication Date: 2006 Jul |
Date Detail:
|
Created Date: 2006-07-11 Completed Date: 2006-08-08 Revised Date: 2009-11-11 |
Medline Journal Info:
|
Nlm Unique ID: 0012737 Medline TA: IEEE Trans Biomed Eng Country: United States |
Other Details:
|
Languages: eng Pagination: 1401-8 Citation Subset: IM |
Affiliation:
|
Institute of Biomedical Engineering, Chung Yuan Christian University, Taiwan 320, ROC. wei20208@tipo.gov.tw |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biosensing Techniques
/
instrumentation*,
methods Electrochemistry / instrumentation*, methods Equipment Design Equipment Failure Analysis Hydrogen-Ion Concentration Microelectrodes Pilot Projects Reproducibility of Results Sensitivity and Specificity Urate Oxidase / analysis, chemistry* Uric Acid / urine* Urinalysis / instrumentation*, methods |
| Chemical | |
Reg. No./Substance:
|
69-93-2/Uric Acid; EC 1.7.3.3/Urate Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Automatic tracing of vocal-fold motion from high-speed digital images.
Next Document: In vivo results of a new focal tissue ablation technique: irreversible electroporation.