Document Detail


Preliminary investigation on the cytotoxicity of tellurite to cultured HeLa cells.
MedLine Citation:
PMID:  12195732     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytotoxicity of tellurite to cultured HeLa cells was examined by cell viability, lactate dehydrogenase (LDH) assay, and tellurite uptake. The experimental results show that the toxicity of tellurite depends on its concentrations and exposure time. HeLa cells exposed to tellurite for 2 h at 9.1 x 10(-4) to 4.5 x 10(-3) mmol/L did not exhibit cytotoxic effects as measured by cell viability. Exposure to tellurite for 24 h at the same concentrations markedly reduced the cell viability to 57% of the control during the first 5 minutes. Additionally, HeLa cells incubated at 2.7 x 10(-2) to 0.27 mmol/L of tellurite for 2 h retained 53% to 67% of cell viability. Even after 24 h exposure, the HeLa cells incubated at 9.1 x 10(-4) to 4.5 x 10(-2) mmol/L of tellurite still retained 57% to 66% of cell viability. Furthermore, tellurite toxicity was also demonstrated in supernatant of the culture at 37 degrees C by LDH assay. It was found that exposure to tellurite for 90 minutes did not stimulate LDH activity. However, tellurite uptake seems to be more sensitive than the cell viability and LDH activity release tests, as it significantly increases with the increasing of exposure time.
Authors:
Wen Jun Ding; Tatsuya Hasegawa; Duan Peng; Hitomi Hosaka; Yoshiyuki Seko
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)     Volume:  16     ISSN:  0946-672X     ISO Abbreviation:  J Trace Elem Med Biol     Publication Date:  2002  
Date Detail:
Created Date:  2002-08-27     Completed Date:  2003-04-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9508274     Medline TA:  J Trace Elem Med Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  99-102     Citation Subset:  IM    
Affiliation:
Department of Environmental Biochemistry, Yamanashi Institute of Environmental Sciences, 5597-1, Kenmarubi, Kamiyoshida, Fujiyoshida, Yamanashi 403-0005, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Survival / drug effects*
Dose-Response Relationship, Drug
Hela Cells
Humans
L-Lactate Dehydrogenase / metabolism
Tellurium / metabolism,  toxicity*
Chemical
Reg. No./Substance:
10049-23-7/tellurous acid; 13494-80-9/Tellurium; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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