Document Detail


Preliminary investigation of 2-methoxyestradiol inducing differentiation of myeloma cell line CZ-1.
MedLine Citation:
PMID:  16584594     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was aimed to investigate whether 2-methoxyestradiol (2ME2) could exert effect of inducing differentiation on myeloma cells. A myeloma cell line CZ-1 secreting lambda light chain protein was used as an object of study. The CZ-1 cell morphology was observed by Wright's staining, the CD49e expression on cell surface after treatment with 2ME2 was detected by flow cytometry, the concentration of lambda light chain protein in the supernatant was assayed by immuno-scattering turbidity method. The results showed that treatments with 0.1-0.5 micromol/L 2ME2 for 72 hours resulted in some mature morphological changes of CZ-1 cells, such as the ratio of karyoplasms going down, nucleolus reducing or disappearing, chromatin getting rougher and more compacted; the CD49e positive CZ-1 cells increased by 2ME2 with concentrations of 0.1 micromol/L to 0.5 micromol/L in a concentration-dependent manner. The statistical difference from the control group was significant; the concentration of lambda light chain protein increased from control group 29.3 +/- 2.77 microg/ml to 35.97 +/- 2.6 microg/ml (P < 0.05) after exposure to 0.1 micromol/L 2ME2 for 72 hours, and the treatment of 0.5 micromol/L 2ME2 up-regulated lambda light chain protein to 79.67 +/- 1.88 microg/ml (P < 0.01) continuously. It is concluded that 2ME2 at low-concentration can induce differentiation of the CZ-1 cells to mature, which provides a new, and safe strategy for myeloma therapy.
Authors:
Jian Hou; Hong Xiong; Wei-Ran Gao; Hua Jiang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology     Volume:  14     ISSN:  1009-2137     ISO Abbreviation:  Zhongguo Shi Yan Xue Ye Xue Za Zhi     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-04-04     Completed Date:  2007-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101084424     Medline TA:  Zhongguo Shi Yan Xue Ye Xue Za Zhi     Country:  China    
Other Details:
Languages:  eng     Pagination:  65-9     Citation Subset:  IM    
Affiliation:
Department of Hematology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China. houjian167@sohu.com
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cell Transformation, Neoplastic / drug effects*
Estradiol / analogs & derivatives*,  pharmacology
Humans
Immunoglobulin lambda-Chains / biosynthesis*
Integrin alpha3 / biosynthesis*
Multiple Myeloma / metabolism,  pathology*
Chemical
Reg. No./Substance:
0/Immunoglobulin lambda-Chains; 0/Integrin alpha3; 362-07-2/2-methoxyestradiol; 50-28-2/Estradiol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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