Document Detail

Preliminary findings of quorum signal molecules in clinically stable lung allograft recipients.
MedLine Citation:
PMID:  12728169     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Infection with bacteria such as Pseudomonas is common in lung allograft recipients, particularly during chronic rejection. Analysis of sputum samples from patients with cystic fibrosis infected with Pseudomonas aeruginosa or Burkholderia cepacia has indicated the presence of bacterial N-acylhomoserine lactones (AHLs) quorum sensing signalling molecules. AHLs not only control the expression of bacterial virulence genes but are also involved in stimulating the maturation of antibiotic resistant biofilms and host chemokine release. It was hypothesised that AHLs may be detected even in clinically stable lung transplant recipients free of clinical infection or rejection. METHODS: Three 60 ml samples of bronchoalveolar lavage (BAL) fluid were taken from nine stable lung transplant recipients 3-12 months after transplantation. Detection of AHLs was carried out on dichloromethane extracted supernatants using the bioluminescence based AHL reporter plasmid pSB1075. This responds to the presence of AHLs with long acyl chains (C10-C14), generating light. Synthetic AHLs were included as positive controls. RESULTS: Five of the nine BAL fluid supernatants exhibited AHL activity, suggesting the presence of AHLs with long N-acyl chains. There was no correlation between the levels of AHLs detected or their absence and BAL fluid microbiology or diagnosis before transplantation. CONCLUSIONS: This is the first evidence for the presence of AHL quorum sensing signals in human lung allograft recipients, even in subjects with no rejection or apparent infection. Further longitudinal follow up of these preliminary findings is required to elucidate potential links with infection, rejection, and allograft deterioration.
C Ward; M Cámara; I Forrest; R Rutherford; G Pritchard; M Daykin; A Hardman; A de Soyza; A J Fisher; P Williams; P A Corris
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thorax     Volume:  58     ISSN:  0040-6376     ISO Abbreviation:  Thorax     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-02     Completed Date:  2003-07-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0417353     Medline TA:  Thorax     Country:  England    
Other Details:
Languages:  eng     Pagination:  444-6     Citation Subset:  IM    
ImmunoBiology and Transplantation Group, University of Newcastle upon Tyne and The Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK.
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MeSH Terms
Bronchoalveolar Lavage Fluid / chemistry*
Burkholderia Infections / complications
Cystic Fibrosis / microbiology*,  surgery
Ligases / analysis*
Lung Transplantation*
Pseudomonas Infections / complications
Sputum / microbiology
Transplantation, Homologous
Reg. No./Substance:
EC 6.-/Ligases; EC 6.1.-/N-acylhomoserine lactone synthase

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