| Preliminary data on the establishment of permanent cell lines continuously producing HIV-1: factors affecting the transmissibility and adaptability of "wild" HIV-1 isolates. | |
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MedLine Citation:
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PMID: 8469168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examines some technical aspects of the transmission to and permanent adaptability in continuous cell lines of wild HIV-1 isolates. Three cell systems (the lymphocytic cell lines Molt-3 and H-9 and the monocytoid cell line U-937) and two transmission protocols (cell to cell and cell-free) were used. Two different replicative behaviours were observed among isolates efficiently transmitted: a) transmissibility but not adaptability (consisting in a limited length of viral replication); aa) transmissibility and adaptability (consisting in a stable and long term virus production). The second type of replication was confined to viruses from patients with severe immunodeficiency. Technical and viral factors can affect the rate of transmissibility and adaptability: the modality of infection (cell to cell transmission appeared to be the most efficient) and the tropism of the virus (some viruses could infect only one T cell line). |
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Authors:
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M Di Stefano; J R Fiore; G Angarano; C Fico; A Grottola; G Pastore |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The new microbiologica Volume: 16 ISSN: 1121-7138 ISO Abbreviation: New Microbiol. Publication Date: 1993 Jan |
Date Detail:
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Created Date: 1993-05-11 Completed Date: 1993-05-11 Revised Date: 2011-02-25 |
Medline Journal Info:
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Nlm Unique ID: 9516291 Medline TA: New Microbiol Country: ITALY |
Other Details:
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Languages: eng Pagination: 19-26 Citation Subset: IM; X |
Affiliation:
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Clinic of Infectious Diseases, Policlinico Hospital, Bari, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Cytopathogenic Effect, Viral HIV Core Protein p24 / biosynthesis HIV-1 / physiology* Humans Tumor Cells, Cultured / microbiology* Virus Replication* |
| Chemical | |
Reg. No./Substance:
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0/HIV Core Protein p24 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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