Document Detail


Preliminary data on the establishment of permanent cell lines continuously producing HIV-1: factors affecting the transmissibility and adaptability of "wild" HIV-1 isolates.
MedLine Citation:
PMID:  8469168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study examines some technical aspects of the transmission to and permanent adaptability in continuous cell lines of wild HIV-1 isolates. Three cell systems (the lymphocytic cell lines Molt-3 and H-9 and the monocytoid cell line U-937) and two transmission protocols (cell to cell and cell-free) were used. Two different replicative behaviours were observed among isolates efficiently transmitted: a) transmissibility but not adaptability (consisting in a limited length of viral replication); aa) transmissibility and adaptability (consisting in a stable and long term virus production). The second type of replication was confined to viruses from patients with severe immunodeficiency. Technical and viral factors can affect the rate of transmissibility and adaptability: the modality of infection (cell to cell transmission appeared to be the most efficient) and the tropism of the virus (some viruses could infect only one T cell line).
Authors:
M Di Stefano; J R Fiore; G Angarano; C Fico; A Grottola; G Pastore
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The new microbiologica     Volume:  16     ISSN:  1121-7138     ISO Abbreviation:  New Microbiol.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-05-11     Completed Date:  1993-05-11     Revised Date:  2011-02-25    
Medline Journal Info:
Nlm Unique ID:  9516291     Medline TA:  New Microbiol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  19-26     Citation Subset:  IM; X    
Affiliation:
Clinic of Infectious Diseases, Policlinico Hospital, Bari, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological
Cytopathogenic Effect, Viral
HIV Core Protein p24 / biosynthesis
HIV-1 / physiology*
Humans
Tumor Cells, Cultured / microbiology*
Virus Replication*
Chemical
Reg. No./Substance:
0/HIV Core Protein p24

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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