Document Detail


Preliminary crystallographic studies of yeast mitochondrial peripheral membrane protein Tim44p.
MedLine Citation:
PMID:  16511294     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein translocations across mitochondrial membranes play critical roles in mitochondrion biogenesis. Protein transport from the cell cytosol to the mitochondrial matrix is carried out by the translocase of the outer membrane (TOM) complex and the translocase of the inner membrane (TIM) complexes. Tim44p is an essential mitochondrial peripheral membrane protein and a major component of the TIM23 translocon. To investigate the mechanism by which Tim44p functions in the TIM23 translocon to deliver the mitochondrial protein precursors, the yeast Tim44p was crystallized. The crystals diffract to 3.2 A using a synchrotron X-ray source and belong to space group P6(3)22, with unit-cell parameters a = 124.25, c = 77.83 A. There is one Tim44p molecule in one asymmetric unit, which corresponds to a solvent content of approximately 43%. Structure determination by MAD methods is under way.
Authors:
Ratnakar Josyula; Zhongmin Jin; Deborah McCombs; Lawrence DeLucas; Bingdong Sha
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-01-27
Journal Detail:
Title:  Acta crystallographica. Section F, Structural biology and crystallization communications     Volume:  62     ISSN:  1744-3091     ISO Abbreviation:  Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-03-02     Completed Date:  2006-05-15     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  101226117     Medline TA:  Acta Crystallogr Sect F Struct Biol Cryst Commun     Country:  England    
Other Details:
Languages:  eng     Pagination:  172-4     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Center for Biophysical Sciences and Engineering, University of Alabama, Birmingham, USA.
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MeSH Terms
Descriptor/Qualifier:
Cloning, Molecular
Crystallization
Crystallography, X-Ray
Escherichia coli / genetics
Intracellular Membranes / chemistry*,  physiology
Membrane Transport Proteins / chemistry*,  genetics,  physiology
Mitochondrial Membrane Transport Proteins / chemistry*,  genetics,  physiology
Protein Precursors / chemistry,  genetics,  physiology
Protein Transport / genetics
Recombinant Proteins / chemistry,  genetics,  metabolism
Saccharomyces cerevisiae Proteins / chemistry*,  genetics,  physiology
Synchrotrons
Grant Support
ID/Acronym/Agency:
R01 DK56203/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/MAS6 protein, S cerevisiae; 0/Membrane Transport Proteins; 0/Mitochondrial Membrane Transport Proteins; 0/Protein Precursors; 0/Recombinant Proteins; 0/Saccharomyces cerevisiae Proteins; 0/TIM44 protein, S cerevisiae
Comments/Corrections

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