| Preliminary crystallographic studies of yeast mitochondrial peripheral membrane protein Tim44p. | |
MedLine Citation:
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PMID: 16511294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protein translocations across mitochondrial membranes play critical roles in mitochondrion biogenesis. Protein transport from the cell cytosol to the mitochondrial matrix is carried out by the translocase of the outer membrane (TOM) complex and the translocase of the inner membrane (TIM) complexes. Tim44p is an essential mitochondrial peripheral membrane protein and a major component of the TIM23 translocon. To investigate the mechanism by which Tim44p functions in the TIM23 translocon to deliver the mitochondrial protein precursors, the yeast Tim44p was crystallized. The crystals diffract to 3.2 A using a synchrotron X-ray source and belong to space group P6(3)22, with unit-cell parameters a = 124.25, c = 77.83 A. There is one Tim44p molecule in one asymmetric unit, which corresponds to a solvent content of approximately 43%. Structure determination by MAD methods is under way. |
Authors:
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Ratnakar Josyula; Zhongmin Jin; Deborah McCombs; Lawrence DeLucas; Bingdong Sha |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-01-27 |
Journal Detail:
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Title: Acta crystallographica. Section F, Structural biology and crystallization communications Volume: 62 ISSN: 1744-3091 ISO Abbreviation: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-03-02 Completed Date: 2006-05-15 Revised Date: 2013-06-07 |
Medline Journal Info:
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Nlm Unique ID: 101226117 Medline TA: Acta Crystallogr Sect F Struct Biol Cryst Commun Country: England |
Other Details:
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Languages: eng Pagination: 172-4 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Center for Biophysical Sciences and Engineering, University of Alabama, Birmingham, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cloning, Molecular Crystallization Crystallography, X-Ray Escherichia coli / genetics Intracellular Membranes / chemistry*, physiology Membrane Transport Proteins / chemistry*, genetics, physiology Mitochondrial Membrane Transport Proteins / chemistry*, genetics, physiology Protein Precursors / chemistry, genetics, physiology Protein Transport / genetics Recombinant Proteins / chemistry, genetics, metabolism Saccharomyces cerevisiae Proteins / chemistry*, genetics, physiology Synchrotrons |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK56203/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/MAS6 protein, S cerevisiae; 0/Membrane Transport Proteins; 0/Mitochondrial Membrane Transport Proteins; 0/Protein Precursors; 0/Recombinant Proteins; 0/Saccharomyces cerevisiae Proteins; 0/TIM44 protein, S cerevisiae |
| Comments/Corrections | |
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