| Preliminary crystallographic studies on bacterioferritin from Azotobacter vinelandii. | |
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MedLine Citation:
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PMID: 6528285 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacterioferritin-cytochrome from Azotobacter vinelandii is an unusual protein containing haem groups as well as iron core like other ferritin. This paper reports the purification of bacterioferritin by affinity chromatography and the formation of brick-red crystals from a solution containing MgCl2. The crystals are optical isotropic with maximum dimensions of 0.4 X 0.4 X 0.1 mm3. The preliminary X-ray crystallographic studies have been performed. 1.5 degrees unscreened precession photographs show that the crystals of bacterioferritin belong to the cubic system, space group I432, with cell dimension 230 A. There are probably 8 molecules in one cubic unit cell and the molecule might have 32 symmetry. A molecular diameter of 115 A is derived from the packing of the molecules and a molecular weight of 826,000 is estimated for bacterioferritin. |
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Authors:
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B G Zhao; Z J Lin; Y Q Tang; J D Li; J W Wang; Y Tu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Scientia Sinica. Series B, Chemical, biological, agricultural, medical & earth sciences / Chung-kuo k'o hsüeh yüan, chu pan Volume: 27 ISSN: 0253-5823 ISO Abbreviation: Sci. Sin., Ser. B, Chem. Biol. Agric. Med. Earth Sci. Publication Date: 1984 Oct |
Date Detail:
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Created Date: 1985-04-11 Completed Date: 1985-04-11 Revised Date: 2009-05-29 |
Medline Journal Info:
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Nlm Unique ID: 8209875 Medline TA: Sci Sin B Country: CHINA |
Other Details:
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Languages: eng Pagination: 1002-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Azotobacter
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analysis* Bacterial Proteins* Chromatography, Affinity Crystallization Crystallography Cytochrome b Group* Ferritins* / isolation & purification* Magnesium Magnesium Chloride Molecular Conformation Molecular Weight |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Cytochrome b Group; 7439-95-4/Magnesium; 7786-30-3/Magnesium Chloride; 9007-73-2/Ferritins; 9035-38-5/bacterioferritin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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