Document Detail


Preliminary crystallographic studies on bacterioferritin from Azotobacter vinelandii.
MedLine Citation:
PMID:  6528285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacterioferritin-cytochrome from Azotobacter vinelandii is an unusual protein containing haem groups as well as iron core like other ferritin. This paper reports the purification of bacterioferritin by affinity chromatography and the formation of brick-red crystals from a solution containing MgCl2. The crystals are optical isotropic with maximum dimensions of 0.4 X 0.4 X 0.1 mm3. The preliminary X-ray crystallographic studies have been performed. 1.5 degrees unscreened precession photographs show that the crystals of bacterioferritin belong to the cubic system, space group I432, with cell dimension 230 A. There are probably 8 molecules in one cubic unit cell and the molecule might have 32 symmetry. A molecular diameter of 115 A is derived from the packing of the molecules and a molecular weight of 826,000 is estimated for bacterioferritin.
Authors:
B G Zhao; Z J Lin; Y Q Tang; J D Li; J W Wang; Y Tu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scientia Sinica. Series B, Chemical, biological, agricultural, medical & earth sciences / Chung-kuo k'o hsüeh yüan, chu pan     Volume:  27     ISSN:  0253-5823     ISO Abbreviation:  Sci. Sin., Ser. B, Chem. Biol. Agric. Med. Earth Sci.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-04-11     Completed Date:  1985-04-11     Revised Date:  2009-05-29    
Medline Journal Info:
Nlm Unique ID:  8209875     Medline TA:  Sci Sin B     Country:  CHINA    
Other Details:
Languages:  eng     Pagination:  1002-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Azotobacter / analysis*
Bacterial Proteins*
Chromatography, Affinity
Crystallization
Crystallography
Cytochrome b Group*
Ferritins* / isolation & purification*
Magnesium
Magnesium Chloride
Molecular Conformation
Molecular Weight
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Cytochrome b Group; 7439-95-4/Magnesium; 7786-30-3/Magnesium Chloride; 9007-73-2/Ferritins; 9035-38-5/bacterioferritin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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