Document Detail


Preliminary assessment of somatic cell nuclear transfer in the dromedary (Camelus dromedarius).
MedLine Citation:
PMID:  18675449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Somatic cloning may enable the maintenance/expansion of the population of camels with the highest potential for milk production or the best racing performances. However, there have been no reports of embryonic or somatic nuclear transfer in camels. The aim of this study was to produce dromedary embryos by nuclear transfer using in vitro matured oocytes and two somatic cells from two sources (adult fibroblasts or granulosa cells). A total of 58 adult females were superstimulated by a single dose of eCG (3500 IU). Ten days later, their ovaries were collected postmortem. Cumulus-oocytes-complexes (COCs) were aspirated from stimulated follicles and were matured in vitro for 30 h. Fibroblasts (from live adult male) and granulosa cells (from slaughtered adult females) were used as donor karyoplasts and injected into mature enucleated dromedary oocytes. The cleavage rate was significantly higher (P<0.05) for embryos reconstructed with fibroblasts (59%) versus those with granulosa cells (45%). However, there was no difference between the two groups in the proportion of cloned embryos reaching the blastocyst stage (fibroblasts: 14% vs. granulosa cells: 15%) or those that hatched (fibroblasts: 10% vs. granulosa cells: 12%). The viability of reconstructed dromedary embryos from the two sources of donor cells (fibroblasts; n=5 vs. granulosa cells; n=7) was examined by transferring them to synchronized recipients. Two females (fibroblasts: 1/5; 20%, granulosa cells: 1/7; 14%) were confirmed pregnant by ultrasonography at 15 and 25 days following transfer. Later, the pregnancies were followed by pregnancy empirical-symptoms. These two pregnancies were lost between 25 and 60 days following transfer, respectively. In conclusion, the present study shows for the first time that the development of dromedary NT embryos derived from either adult fibroblasts or granulosa cells can occur in vitro and the transfer of these cloned embryos to recipients can result in pregnancies.
Authors:
H Khatir; A Anouassi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Theriogenology     Volume:  70     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-14     Completed Date:  2009-03-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1471-7     Citation Subset:  IM    
Affiliation:
Veterinary Research Center, P.O. Box 44857, Abu Dhabi, United Arab Emirates. khatir67@emirates.net.ae
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MeSH Terms
Descriptor/Qualifier:
Animals
Camels / physiology*
Cloning, Organism / methods,  veterinary*
Embryo, Mammalian
Female
Nuclear Transfer Techniques / veterinary*
Ovary
Superovulation

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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