Document Detail

Preliminary investigation of trypanosomosis in exotic dog breeds from Zambia's Luangwa and Zambezi valleys using LAMP.
MedLine Citation:
PMID:  23716412     Owner:  NLM     Status:  MEDLINE    
Abstract. Canine African trypanosomosis (CAT) is rarely reported in the literature. In this preliminary study, we evaluated the performance of loop-mediated isothermal amplification (LAMP) against microscopy to detect CAT in six exotic dog breeds naturally infected with trypanosomes from Zambia's South Luangwa National Park and Chiawa Game Management Area. To our knowledge, this is the first report of CAT in Zambia. The patients exhibited a variety of aspecific clinical signs. The LAMP did not only confirm all six parasitologically positive CAT cases detected passively between April 2010 and January 2012, but was also critical in trypanosome speciation. According to LAMP, the majority of the dogs had monolytic infections with either Trypanosoma congolense or Trypanosoma brucei rhodesiense. The LAMP is thus a potential simple and cost-effective tool for trypanosome diagnosis in endemic regions. The rare report of zoonotic trypanosomes in dogs in Zambia has public health implications and justifies further investigations of CAT.
Boniface Namangala; Elizabeth Oparaocha; Kiichi Kajino; Kyoko Hayashida; Ladslav Moonga; Noboru Inoue; Yasuhiko Suzuki; Chihiro Sugimoto
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-05-28
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  89     ISSN:  1476-1645     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-07-11     Completed Date:  2013-09-16     Revised Date:  2014-07-13    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  116-8     Citation Subset:  AIM; IM    
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MeSH Terms
Dog Diseases / diagnosis,  epidemiology,  parasitology*
Dogs / parasitology
Nucleic Acid Amplification Techniques / methods,  veterinary
Trypanosoma brucei rhodesiense
Trypanosoma congolense
Trypanosomiasis, African / diagnosis,  epidemiology,  veterinary*
Zambia / epidemiology

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