Document Detail

Preincubation with creatine enhances levels of creatine phosphate and prevents anoxic damage in rat hippocampal slices.
MedLine Citation:
PMID:  7760049     Owner:  NLM     Status:  MEDLINE    
We have investigated the relationship between energy metabolism, NMDA-receptor antagonism, and anoxic damage in vitro. Anoxic damage was assessed by measuring protein synthesis, defined as the incorporation of [14C]lysine into perchloric acid-insoluble tissue extracts. The concentrations of energy metabolites were measured by ion-exchange HPLC. Anoxia caused an inhibition of protein synthesis, a reduction in phosphocreatine and adenosine triphosphate, and extensive neuronal damage. The reduction of protein synthesis depended on the duration of anoxia and the time allowed for recovery. Preincubation with the creatine dose-dependently (0.03-3 mmol/L) increased baseline levels of phosphocreatine, reduced the anoxia-induced decline in phosphocreatine and adenosine triphosphate, prevented the impairment of protein synthesis, and reduced neuronal death. Incubation with (R,S)-3-guanidinobutyric acid, a synthetic analogue of creatine that cannot be phosphorylated, did not prevent the anoxia-induced impairment of protein synthesis and did not enhance the levels of phosphocreatine and adenosine triphosphate. Incubation with a combination of both creatine and the noncompetitive NMDA antagonist MK-801 provided complete protection. These results indicate that energy status is a major factor controlling anoxic damage in the rat hippocampal slice.
A J Carter; R E Müller; U Pschorn; W Stransky
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  64     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-06-28     Completed Date:  1995-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2691-9     Citation Subset:  IM    
Department of Biological Research, Boehringer Ingelheim KG, Ingelheim, Germany.
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MeSH Terms
Anoxia / metabolism,  pathology*
Creatine / antagonists & inhibitors,  pharmacology*
Energy Metabolism / drug effects
Guanidines / pharmacology
Hippocampus / drug effects,  metabolism*,  pathology*
Neuroprotective Agents / pharmacology*
Phosphocreatine / metabolism*
Protein Biosynthesis
Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
Reg. No./Substance:
0/Guanidines; 0/Neuroprotective Agents; 0/Receptors, N-Methyl-D-Aspartate; 352-83-0/beta-guanidinobutyric acid; 57-00-1/Creatine; 67-07-2/Phosphocreatine

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