| Preincubation with creatine enhances levels of creatine phosphate and prevents anoxic damage in rat hippocampal slices. | |
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MedLine Citation:
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PMID: 7760049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated the relationship between energy metabolism, NMDA-receptor antagonism, and anoxic damage in vitro. Anoxic damage was assessed by measuring protein synthesis, defined as the incorporation of [14C]lysine into perchloric acid-insoluble tissue extracts. The concentrations of energy metabolites were measured by ion-exchange HPLC. Anoxia caused an inhibition of protein synthesis, a reduction in phosphocreatine and adenosine triphosphate, and extensive neuronal damage. The reduction of protein synthesis depended on the duration of anoxia and the time allowed for recovery. Preincubation with the creatine dose-dependently (0.03-3 mmol/L) increased baseline levels of phosphocreatine, reduced the anoxia-induced decline in phosphocreatine and adenosine triphosphate, prevented the impairment of protein synthesis, and reduced neuronal death. Incubation with (R,S)-3-guanidinobutyric acid, a synthetic analogue of creatine that cannot be phosphorylated, did not prevent the anoxia-induced impairment of protein synthesis and did not enhance the levels of phosphocreatine and adenosine triphosphate. Incubation with a combination of both creatine and the noncompetitive NMDA antagonist MK-801 provided complete protection. These results indicate that energy status is a major factor controlling anoxic damage in the rat hippocampal slice. |
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Authors:
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A J Carter; R E Müller; U Pschorn; W Stransky |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of neurochemistry Volume: 64 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-06-28 Completed Date: 1995-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2691-9 Citation Subset: IM |
Affiliation:
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Department of Biological Research, Boehringer Ingelheim KG, Ingelheim, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia / metabolism, pathology* Creatine / antagonists & inhibitors, pharmacology* Energy Metabolism / drug effects Guanidines / pharmacology Hippocampus / drug effects, metabolism*, pathology* Male Neuroprotective Agents / pharmacology* Phosphocreatine / metabolism* Protein Biosynthesis Rats Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors |
| Chemical | |
Reg. No./Substance:
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0/Guanidines; 0/Neuroprotective Agents; 0/Receptors, N-Methyl-D-Aspartate; 352-83-0/beta-guanidinobutyric acid; 57-00-1/Creatine; 67-07-2/Phosphocreatine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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