| Preimplantation mouse embryos express a heat-stable alkaline phosphatase. | |
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MedLine Citation:
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PMID: 2511934 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cell surface alkaline phosphatase (APase) of the preimplantation mouse embryo has been characterized in situ by inhibition studies and fluorescent histochemistry. The embryonic APase has also been compared in the inhibition studies to the APase expressed on mouse F9 teratocarcinoma cells and, in some instances, to the APase of mouse intestinal epithelial cells. The embryonic APase was active over the pH range of 6.0-10.0, with the optimal pH for full activity in the range of 8.5-10.0. The embryonic APase was remarkably heat stable with significant loss of activity detected only after a 1 h incubation at 90 degrees C. A variety of specific and nonspecific APase inhibitors were applied to embryos to determine the nature of the APase isozymes expressed on these cells. The embryonic APase was totally resistant to levamisole, tetramisole, bromotetramisole, and L-homoarginine. The embryonic APase was inhibited by L-phenylalanine in a concentration-dependent fashion and by sodium arsenate, sodium vanadate, ethylenediamine tetraacetic acid, and slightly by 1,10-phenanthroline. The inhibition profile of the mouse embryonic APase, therefore, resembles most closely that reported for human placental APase with respect to heat stability and that reported for mouse intestinal APase with respect to inhibitor sensitivity. |
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Authors:
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M L Lepire; C A Ziomek |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biology of reproduction Volume: 41 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1990-01-25 Completed Date: 1990-01-25 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 464-73 Citation Subset: IM |
Affiliation:
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Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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antagonists & inhibitors,
metabolism* Animals Blastocyst / enzymology* Edetic Acid / pharmacology Enzyme Stability Female Hot Temperature Hydrogen-Ion Concentration Metals / pharmacology Mice Phenanthrolines / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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HD 17674/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Metals; 0/Phenanthrolines; 60-00-4/Edetic Acid; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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