Document Detail

Preimplantation genetic diagnosis for fetal neonatal alloimmune thrombocytopenia due to antihuman platelet antigen maternal antibodies.
MedLine Citation:
PMID:  22270286     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To develop a reliable preimplantation genetic diagnosis protocol for antihuman platelet antigen-1 incompatibility for a family in whom antenatal treatment was not possible because of the mother's hypersensitivity to intravenous immunoglobulin (IVIG).
METHODS: Haplotypes were constructed from genomic DNA of the family members. A polymerase chain reaction protocol that included eight microsatellite polymorphic markers and the ITGB3-specific (T196C, rs5918) polymorphism were multiplexed to be used in a single cell protocol, and single blastomeres were analyzed.
RESULTS: In one preimplantation genetic diagnosis cycle, out of 28 retrieved oocytes, 24 embryos fertilized and 12 underwent biopsy. Three embryos were found to be antihuman platelet antigen-1b/1b homozygotes and two were transferred. This cycle resulted in an uneventful pregnancy and birth of a healthy child.
CONCLUSION: In cases in which there is antihuman platelet antigen incompatibility and IVIG cannot be administered, preimplantation genetic diagnosis is a reliable alternative to enable birth of unaffected children.
Gheona Altarescu; T Eldar-Geva; T Eldar Geva; Sorina Grisaru-Granovsky; Lilach Bonstein; Hagit Miskin; Irit Varshver; Ehud J Margalioth; Ephrat Levy-Lahad; Paul Renbaum
Related Documents :
6499496 - The effect of cooling rate and warming rate on the packing effect in human erythrocytes...
10638696 - Thrombin receptor activating peptide (sfllrn) potentiates shear-induced platelet microv...
2858226 - Inescapable shock, neurotransmitters, and addiction to trauma: toward a psychobiology o...
12941046 - Different effects of various anti-gpiib-iiia agents on shear-induced platelet activatio...
8385286 - Enhanced platelet reactivity with erythropoietin but not following transfusion in dialy...
14581086 - Coagulation factor xa synergistically interacts with serotonin in inducing vascular smo...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Obstetrics and gynecology     Volume:  119     ISSN:  1873-233X     ISO Abbreviation:  Obstet Gynecol     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-24     Completed Date:  2012-06-11     Revised Date:  2012-06-29    
Medline Journal Info:
Nlm Unique ID:  0401101     Medline TA:  Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  338-43     Citation Subset:  AIM; IM    
Medical Genetics Institute, ZOHAR Preimplantation Genetic Diagnosis Unit, Department of Obstetrics and Gynecology, Shaare Zedek Medical Center, Hebrew University Medical School, Jerusalem, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, Human Platelet / genetics*,  immunology
Fertilization in Vitro
Genetic Testing*
Immunoglobulins, Intravenous / contraindications,  immunology
Infant, Newborn
Integrin beta3 / genetics*
Live Birth
Microsatellite Repeats
Polymerase Chain Reaction
Polymorphism, Genetic
Preimplantation Diagnosis / methods*
Thrombocytopenia, Neonatal Alloimmune / diagnosis*,  genetics,  prevention & control
Reg. No./Substance:
0/Antigens, Human Platelet; 0/ITGB3 protein, human; 0/Immunoglobulins, Intravenous; 0/Integrin beta3
Erratum In:
Obstet Gynecol. 2012 Apr;119(4):872
Note: Geva, T Eldar [corrected to Eldar-Geva, T]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Single-Incision Mini-Sling Compared With Tension-Free Vaginal Tape for the Treatment of Stress Urina...
Next Document:  Maternal cardiac disease: update for the clinician.