Document Detail

Pregrowth-desiccation: a simple and efficient procedure for the cryopreservation of rice (Oryza sativa L.) embryogenic suspension cells.
MedLine Citation:
PMID:  11788862     Owner:  NLM     Status:  MEDLINE    
Rice embryogenic suspension cells were successfully cryopreserved by a pregrowth-desiccation procedure. Cells were precultured in liquid AA medium containing 0.175 mol/L sucrose for 3 d and then in liquid AA medium containing 0.4 mol/L sorbitol for 1 d. After air-drying for about 20 h to a water content of 10%, the cells were placed into cryotubes and quenched into liquid nitrogen. Using this pregrowth-desiccation procedure, a survival rate of 96+/-6% (TTC reduction assay) or 100% (cell clump regrowth) was achieved. Cryostored cells revived very quickly during the recovery culture and they retained the ability to regenerate fertile plants. In conclusion, air-drying, a method usually employed in cryopreservation of seeds or shoot tips, can be used as a simple and efficient procedure for the cryopreservation of precultured rice suspension cells.
Y X Zhang; J H Wang; H W Bian; M Y Zhu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  22     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2001 Jul-Aug
Date Detail:
Created Date:  2002-01-14     Completed Date:  2002-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  221-8     Citation Subset:  IM    
College of Life Sciences, Zhejiang University, Hangzhou 310012, China.
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MeSH Terms
Cryopreservation / methods*
Culture Media
Oryza sativa / growth & development*
Seeds / cytology*
Reg. No./Substance:
0/Culture Media

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