Document Detail

Pregnancy outcome in mares following insemination deep in the uterine horn with low numbers of sperm selected by glass wool/Sephadex filtration, Percoll separation or absolute number.
MedLine Citation:
PMID:  12853183     Owner:  NLM     Status:  MEDLINE    
Mares were inseminated deep in the uterine horn with 25 million sperm selected by glass wool/Sephadex (GWS) filtration, Percoll separation (PS) or absolute number (AN). Deep-horn insemination using a low-volume, smooth tipped, flexible pipette/catheter delivery system allowed more efficient use of stallion sperm and reduced post-breeding uterine reaction in mares. Mares were pregnant in 15/30, 13/30 and 10/30 cycles for GWS, PS and AN selection methods, respectively. Sperm selection method did not effect pregnancy outcome (P=0.422). However, sperm selected for deep-horn insemination by filtration through a glass wool/Sephadex column tended to improve fertility over simply using an absolute number of sperm (P=0.105).
Gary J Nie; Kristina E Johnson; James G W Wenzel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Animal reproduction science     Volume:  79     ISSN:  0378-4320     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-07-10     Completed Date:  2003-11-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  103-9     Citation Subset:  IM    
Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, AL 36849-5522, USA.
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MeSH Terms
Cell Separation / methods*
Centrifugation, Density Gradient
Chromatography, Gel
Insemination, Artificial / methods,  veterinary*
Pregnancy Outcome / veterinary*
Silicon Dioxide
Sperm Count*
Spermatozoa / cytology
Reg. No./Substance:
65455-52-9/Percoll; 7631-86-9/Silicon Dioxide; 9003-39-8/Povidone; 9004-54-0/Dextrans; 9014-76-0/sephadex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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