Document Detail

Pregnancy and live birth following the transfer of vitrified-warmed blastocysts derived from zona- and corona-cell-free oocytes.
MedLine Citation:
PMID:  20800550     Owner:  NLM     Status:  MEDLINE    
This study reports two clinical pregnancies and one live birth following the transfer of vitrified blastocysts developed from oocytes with neither zona pellucida nor corona cells. Two zona-free oocytes obtained from two patients of advanced maternal age undergoing minimal stimulation were normally fertilized after intracytoplasmic sperm injection. In case 1, all four blastomeres of the zona-free embryo were loosely associated and inserted back into ruptured zona on day 2. Zona-free embryo from case 2 had tight contacts between blastomeres and was cultured without zona. Both embryos derived from zona-free oocytes progressed to blastocyst stage and were cryopreserved by vitrification. When patients came back for a cryopreserved embryo transfer, both vitrified blastocysts survived warming. In case 1, transfer of a warmed blastocyst with reconstructed zona resulted in a clinical pregnancy that ended in a spontaneous abortion at 22 weeks. In case 2, live birth was achieved with a normal healthy baby (male) weighing 2381 g at 40 weeks' gestation. This report emphasizes the importance of maintenance of normal cell arrangement on the subsequent embryonic development for a zona-free oocyte. Zona-free oocytes may provide a valuable source of embryos for infertility patients, especially for those with a limited number of oocytes.
Yimin Shu; Wenlin Peng; John Zhang
Publication Detail:
Type:  Case Reports; Journal Article     Date:  2010-05-10
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  21     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  527-32     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
New Hope Fertility Center, 784 Park Avenue, New York City, NY 10021, USA; IVF Program, Stanford University Medical Center, Palo Alto, CA 94304, USA.
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MeSH Terms
Blastocyst / physiology*
Cryopreservation / methods
Embryo Transfer / methods*
Live Birth
Maternal Age
Oocytes / growth & development
Semen Preservation
Sperm Injections, Intracytoplasmic
Zona Pellucida / physiology*

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