Document Detail

Pregnancy induces expression of cPLA2 in ovine uterine artery but not systemic artery endothelium.
MedLine Citation:
PMID:  10643582     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine whether pregnancy increases cytosolic phospholipase A2 (cPLA2) expression in uterine artery (UA) endothelial cells and vascular smooth-muscle (VSM) cells, and whether ovarian steroids mediate this effect. METHODS: Uterine arteries and omental arteries (systemic control) were isolated from pregnant (120-130 days' gestation) ewes and from nonpregnant ewes synchronized to the corresponding phases of the ovarian cycle (follicular or luteal) or ovariectomized. In addition, ovariectomized ewes were treated with vehicle, estradiol-17 beta (E2 beta), progesterone (P4), or combined E2 beta/P4 for 10 days, and UAs were collected. Arteries from all studies were separated into mechanically isolated endothelial and VSM fractions. Proteins were then solubilized and separated on 7.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western immunoblotting using an affinity-purified mouse monoclonal antibody for cPLA2. RESULTS: Both UA endothelium and VSM fractions were seen to express cPLA2, detected as a single band with a molecular mass similar to that observed in myometrium (about 100 kD). Distribution of cPLA2, when expressed per microgram of protein, was observed as 40% in UA endothelium compared with 60% in VSM fractions. Uterine artery endothelial cPLA2 expression was specifically increased 1.9-fold in pregnancy (P < .05), whereas there was no significant change from VSM. Furthermore, in ovariectomized sheep versus intact luteal or follicular phase animals, there was no significant change in cPLA2 expression in endothelium or VSM. Administration of E2 beta, P4, or their combination in ovariectomized sheep also failed to reproduce the pregnancy-induced increase in cPLA2 expression in UA endothelium. Omental artery endothelial and VSM cPLA2 expression was observed at similar magnitudes as UA expression, but levels were consistently unchanged by pregnancy, the ovarian cycle, or ovariectomy. CONCLUSIONS: cPLA2 was expressed throughout the endothelium and VSM of both uterine and omental arteries, but only in UA endothelium was pregnancy associated with elevated cPLA2 expression. The lack of change in cPLA2 expression with the ovarian cycle or in ovariectomized animals, even after prolonged treatment with E2 beta, P4, or E2 beta/P4 combined suggests this pregnancy-induced increase in cPLA2 may not be estrogen and/or progesterone dependent.
T Di; J A Sullivan; H L Rupnow; R R Magness; I M Bird
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the Society for Gynecologic Investigation     Volume:  6     ISSN:  1071-5576     ISO Abbreviation:  J. Soc. Gynecol. Investig.     Publication Date:    1999 Nov-Dec
Date Detail:
Created Date:  2000-02-10     Completed Date:  2000-02-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9433806     Medline TA:  J Soc Gynecol Investig     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  301-6     Citation Subset:  IM    
Department of Obstetrics and Gynecology, University of Wisconsin-Madison.
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MeSH Terms
Arteries / enzymology*
Blotting, Western
Endothelium, Vascular / enzymology*
Estradiol / pharmacology
Nitric Oxide Synthase / analysis
Nitric Oxide Synthase Type III
Phospholipases A / analysis*
Phospholipases A2
Pregnancy, Animal / physiology*
Progesterone / pharmacology
Uterus / blood supply*
Grant Support
Reg. No./Substance:
50-28-2/Estradiol; 57-83-0/Progesterone; EC Oxide Synthase; EC Oxide Synthase Type III; EC 3.1.1.-/Phospholipases A; EC A2

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