| Pregnancy-induced fluctuations in functional T-cell subsets in multiple sclerosis patients. | |
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MedLine Citation:
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PMID: 20610493 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: During pregnancy, especially during the third trimester, multiple sclerosis (MS) disease activity is reduced. It is not known which factors mediate this disease amelioration. OBJECTIVE: To study whether the frequency of two important T-cell subsets, T-helper 17 (Th17) and regulatory T-cells (Treg), is altered in relation to pregnancy-induced MS disease amelioration. METHODS: Each individual was tested longitudinally, after sampling of blood at timepoints before pregnancy, during the first and third trimester, and in the early post-partum period. Frequencies of Th17 cells were assessed after short (4 hours) re-stimulation of peripheral blood lymphocytes with PMA and ionomycin, followed by flow cytometry using CD4, CD45RO and IL-17A antibodies. To assess peripheral blood Treg frequencies, we used six-colour flow cytometry with antibodies against CD3, CD4, CD25, CD127, FoxP3 and HLA-DR, to specifically identify Treg. RESULTS: Both MS patients (n = 9) and controls (n = 8) displayed unaltered Th17 frequencies during pregnancy. In contrast, circulating Treg frequency significantly decreased in MS patients (n = 15) during the first and third (p < 0.001) trimesters compared with the period before pregnancy. In the post-partum period, the frequency of circulating Treg again resurged back to near pre-pregnancy levels. In controls (n = 15) comparable frequency kinetics were observed in that post-partum a significant increase in circulating Treg frequency was detected compared with the first (p < 0.001) and third (p = 0.012) trimester. CONCLUSIONS: Third trimester amelioration is not related to the fluctuation of circulating Th17 cells. Furthermore, a paradoxical decrease of immunosuppressive circulating Tregs can be observed during this phase, both in MS patients and controls. |
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Authors:
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Rinze F Neuteboom; Evert Verbraak; Annet F Wierenga-Wolf; Marjan van Meurs; Eric A P Steegers; Christianne J M de Groot; Jon D Laman; Rogier Q Hintzen |
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Publication Detail:
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Type: Journal Article Date: 2010-07-07 |
Journal Detail:
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Title: Multiple sclerosis (Houndmills, Basingstoke, England) Volume: 16 ISSN: 1477-0970 ISO Abbreviation: Mult. Scler. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-03 Completed Date: 2010-12-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9509185 Medline TA: Mult Scler Country: England |
Other Details:
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Languages: eng Pagination: 1073-8 Citation Subset: IM |
Affiliation:
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Department of Neurology, Erasmus MC, Erasmus University Medical Center, Rotterdam, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Case-Control Studies Chi-Square Distribution Disability Evaluation Female Flow Cytometry Humans Immune Tolerance Immunophenotyping Interleukin-17 / metabolism* Linear Models Longitudinal Studies Lymphocyte Count Multiple Sclerosis, Relapsing-Remitting / diagnosis, immunology* Netherlands Pregnancy Pregnancy Complications / diagnosis, immunology* Pregnancy Trimester, Third / immunology Severity of Illness Index T-Lymphocyte Subsets / immunology* T-Lymphocytes, Helper-Inducer / immunology* T-Lymphocytes, Regulatory / immunology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-17 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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