Document Detail

Pregnancy following fertilization of zona-free, coronal cell intact human ova: Case Report.
MedLine Citation:
PMID:  11139556     Owner:  NLM     Status:  MEDLINE    
A pregnancy by intracytoplasmic sperm injection (ICSI) of a couple whose oocytes were recovered without a zona pellucida is reported. Previous IVF/ICSI cycles indicated all oocytes had either very thin or no zona pellucida at the time of cumulus removal prior to ICSI. As a result, many ova were very fragile and lysed upon handling. In the latest attempt, six ova were recovered. After coronal cell dissection of two ova where both lacked a zona pellucida and one lysed, it was elected to attempt to inject the remaining four ova without removal of their coronal cells. The zona pellucida was not visible through the cell layer and the position of the polar body in three ova was speculative. Two embryos possessed normal pronuclei on day 1, and displayed six blastomeres prior to transfer on day 3. During preparation for transfer, sufficient coronal cells fell away to confirm both embryos lacked a zona pellucida. Pregnancy was confirmed by ultrasound observation of a fetal heart 4 weeks later. This report documents that where the zona pellucida is absent, the coronal cells provide sufficient support to maintain blastomere interaction and embryo viability and the zona pellucida is not essential for early embryo development and pregnancy.
J D Stanger; K Stevenson; A Lakmaker; R Woolcott
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  16     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-03-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  164-167     Citation Subset:  IM    
Lingard Fertility Centre, Merewether, Newcastle, Australia.
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MeSH Terms
Embryo Transfer
Embryonic and Fetal Development
Infertility, Female / pathology*,  therapy*
Oligospermia / therapy
Sperm Injections, Intracytoplasmic / methods*
Zona Pellucida / pathology*,  physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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